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Detection of Neuronal and Glial Antigens After Decalcification in Formic Acid Solution and Fixation in Zinc-Ethanol-Formaldehyde

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The aim of the present work was to identify the possibility of immunocytochemical detection of various tissue antigens on paraffin sections of rat spinal cord surrounded by spinal bony tissue and adjacent muscles fixed in zinc-ethanol-formaldehyde after decalcification with formic acid in adult (n = 5) and neonatal (n = 2) Wistar rats. These studies used antibodies to glial fibrillary acidic protein, neurofilaments, neuronal nuclear protein, tyrosine hydroxylase, the synaptic vesicle protein synaptophysin, and the intermediate filament protein vimentin. The combination of fixation in zinc-ethanol-formaldehyde and decalcification in 21% formic acid solution provided optimal preservation of the antigens of interest.

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Authors and Affiliations

  1. Laboratory for the Functional Morphology of the Central and Peripheral Nervous System, Department of General and Special Morphology, Research Institute of Experimental Medicine, North-West Branch, Russian Academy of Medical Sciences, St. Petersburg, Russia

    E. A. Kolos & D. E. Korzhevskii

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  1. E. A. Kolos
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  2. D. E. Korzhevskii
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Correspondence to D. E. Korzhevskii.

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Translated from Morfologiya, Vol. 144, No. 4, pp. 76–79, July–August, 2013.

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Kolos, E.A., Korzhevskii, D.E. Detection of Neuronal and Glial Antigens After Decalcification in Formic Acid Solution and Fixation in Zinc-Ethanol-Formaldehyde. Neurosci Behav Physi 44, 790–792 (2014). https://doi.org/10.1007/s11055-014-9985-3

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  • DOI: https://doi.org/10.1007/s11055-014-9985-3

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