Skip to main content
SpringerLink
Log in

A method of preparing single renal tubules for electron microprobe analysis

  • Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands
  • Instruments and Techniques
  • Published:
Pflügers Archiv Aims and scope Submit manuscript

Abstract

A method is described whereby single renal tubules, incubated in varying incubation media can be captured and prepared for electron microprobe analysis for intracellular sodium, potassium, chloride, phosphorus, magnesium amongst others.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Balaban RS, Soltoff SP, Storey JM, Mandel LJ (1980) Improved renal cortical tubule suspension: Spectrophotometric study of O2 delivery. Am J Physiol 238:F50-F59

    Google Scholar 

  2. Beck F, Bauer R, Bauer U, Mason J, Dörge A, Rick R, Thurau K (1980) Electron microprobe analysis of intracellular elements in the rat kidney. Kidney Int 17:756–763

    Google Scholar 

  3. Dörge A, Rick R, Gehring K, Thurau K (1978) Preparation of freeze-dried cryosections for quantitative X-ray microanalysis of electrolytes in biological soft tissues. Pflügers Arch 373:85–97

    Google Scholar 

  4. Guder WG, Wiesner W, Stukowski B, Wieland OH (1971) Metabolism of isolated kidney tubules. Oxygen consumption, gluconeogenesis and the effect of cyclic nucleotides in tubules from starved rats. Hoppe-Seyler's Z Physiol Chem 352:1319–1328

    Google Scholar 

  5. Gupta BL, Berridge MJ, Hall TA, Moreton RB (1978) Electron microprobe and ion-selective microelectrode studies of fluid secretion in the salivary glands of Calliphora. J Exp Biol 72:261–284

    Google Scholar 

  6. Klein KL Wang MS, Torikai S, Davidson WK, Kurokawa K (1981) Substrate oxidation by isolated single nephron segments of the rat. Kidney Int 20:29–35

    Google Scholar 

  7. Rick R, Dörge A, von Arnim E, Thurau K (1978) Electron microprobe analysis of frog skin epithelium: Evidence for a syncytial sodium transport compartment. J Membrane Biol 39: 313–331

    Google Scholar 

  8. Rick R, Dörge A, Macknight ADC, Leaf A, Thurau K (1978) Electron microprobe analysis of the different epithelial cells of toad urinary bladder. J Membrane Biol 39:257–271

    Google Scholar 

  9. Scholer DW, Edelmann IS (1979) Isolation of rat kidney cortical tubules enriched in proximal and distal segments. Am J Physiol 237:F350-F359

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Medicine, University of Sydney at the Royal North Shore Hospital, 2065, St. Leonards, N.S.W., Australia

    A. Z. Györy

  2. Physiologisches Institut der Universität München, Pettenkoferstraße 12, D-8000, München 2, Federal Republic of Germany

    F. X. Beck & K. Thurau

Authors
  1. A. Z. Györy
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. F. X. Beck
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. K. Thurau
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Györy, A.Z., Beck, F.X. & Thurau, K. A method of preparing single renal tubules for electron microprobe analysis. Pflugers Arch. 399, 241–242 (1983). https://doi.org/10.1007/BF00656723

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00656723

Key words

Search

Navigation