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Sample Preparation and Stereological Methods for the Study of Glomerular Ultrastructure Using Electron Microscopy

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Diabetic Nephropathy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2067))

Abstract

In this chapter we describe conventional methods used for preparing renal tissue for transmission electron microscopy. We also describe a relatively new technique, serial block face scanning electron microscopy. Protocols are given for processing, sectioning, and imaging of tissue along with methods for obtaining quantitative data from the results.

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Author information

Authors and Affiliations

  1. EM Research Services, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne, UK

    Kathryn E. White

Authors
  1. Kathryn E. White
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Correspondence to Kathryn E. White .

Editor information

Editors and Affiliations

  1. Faculty of Life Sciences & Medicine School of Cardiovascular Medicine & Sciences, British Heart Foundation Centre of Research Excellence, King’s College London, London, UK

    Luigi Gnudi

  2. Developmental Biology and Cancer Programme, UCL Great Ormond Street Institute of Child Health, University College London, London, UK

    David A. Long

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White, K.E. (2020). Sample Preparation and Stereological Methods for the Study of Glomerular Ultrastructure Using Electron Microscopy. In: Gnudi, L., Long, D. (eds) Diabetic Nephropathy. Methods in Molecular Biology, vol 2067. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9841-8_7

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  • DOI: https://doi.org/10.1007/978-1-4939-9841-8_7

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9840-1

  • Online ISBN: 978-1-4939-9841-8

  • eBook Packages: Springer Protocols

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