Skip to main content

Artifacts and Pitfalls in Electron Microscopy of the Kidney

Atlas of Renal Ultrastructural Pathology
  • 165 Accesses

Abstract

This chapter will give a brief overview of the main technical artifacts and pitfalls in transmission electron microscopy examination of the kidney, with illustrations.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Institutional subscriptions

References

  1. Walker P, Cavallo T, Bonsib S. Practical guidelines for the renal biopsy. Mod Pathol. 2004;17:1555–63.

    Article  PubMed  Google Scholar 

  2. Tsutsumi Y. Electron microscopic study using formalin-fixed, paraffin-embedded material, with special reference to observation of microbial organisms and endocrine granules. Acta Histochem Cytochem. 2018;51(2):63–71.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Graham L, Orenstein JM. Processing tissue and cells for transmission electron microscopy in diagnostic pathology and research. Nat Protoc. 2007;2:2439–50.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Lavorato M, Franzini-Armstrong C. Practical solutions to frequent problems encountered in thin sections electron microscopy. Microsc Today. 2017;25(3):40–5. https://doi.org/10.1017/S1551929517000438.

    Article  CAS  Google Scholar 

  5. Pandithage R. Brief introduction to contrasting for EM sample preparation. Leica Microsystems. 2013. Available via https://www.leica-microsystems.com/science-lab/brief-introduction-to-contrasting-for-em-sample-preparation/. Accessed 1 Nov 2021.

  6. Yan R, Li K, Jiang W. Defocus and magnification dependent variation of TEM image astigmatism. Sci Rep. 2018;8(1):344. https://doi.org/10.1038/s41598-017-18820-x.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Tracey de Haro .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2023 Springer Nature Switzerland AG

About this entry

Check for updates. Verify currency and authenticity via CrossMark

Cite this entry

de Haro, T. (2023). Artifacts and Pitfalls in Electron Microscopy of the Kidney. In: Roufosse, C., Broecker, V. (eds) Atlas of Renal Ultrastructural Pathology. Springer, Cham. https://doi.org/10.1007/978-3-030-44817-2_6-1

Download citation

  • DOI: https://doi.org/10.1007/978-3-030-44817-2_6-1

  • Received:

  • Accepted:

  • Published:

  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-030-44817-2

  • Online ISBN: 978-3-030-44817-2

  • eBook Packages: Springer Reference MedicineReference Module Medicine

Publish with us

Policies and ethics

Chapter history

  1. Latest

    Artifacts and Pitfalls in Electron Microscopy of the Kidney
    Published:
    29 January 2024

    DOI: https://doi.org/10.1007/978-3-030-44817-2_6-2

  2. Original

    Artifacts and Pitfalls in Electron Microscopy of the Kidney
    Published:
    22 March 2023

    DOI: https://doi.org/10.1007/978-3-030-44817-2_6-1