Summary
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1.
When dorsal root ganglia were incubated in vitro with a range of concentrations of anisomycin, incorporation of [3H]lysine into protein and synthesis of substance P (SP) were inhibited to a similar extent confirming that SP is synthesised by a conventional ribosomal mechanism.
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2.
Doses of anisomycin sufficient to inhibit protein synthesis in mouse CNS in vivo by greater than 95% did not cause a significant fall in the SP content of any of five brain areas, spinal cord, dorsal root ganglia or cornea over an 8 h period.
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3.
Electrical stimulation of the hind limbs however, produced a 25% fall in the SP content of the dorsal spinal cord of these animals.
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4.
These findings suggest that the turnover-time of neuronal SP is much longer than that of non-peptide neurotransmitters.
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Winter, E., Keen, P. Effects of synthesis inhibition and nervous activity on concentrations of neuronal substance P. Naunyn-Schmiedeberg's Arch. Pharmacol. 323, 173–175 (1983). https://doi.org/10.1007/BF00634267
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DOI: https://doi.org/10.1007/BF00634267