Summary
Posttreatment with sodium arsenite in log phase synergistically increases the chromosomal aberrations induced by ethyl methanesulfonate in Chinese hamster ovary cells, human fibroblasts, and human lymphocytes. However, posttreatment with sodium arsenite in stationary phase has no apparent effect on the clastogenicity of ethyl methanesulfonate. These results indicate that the cycling state of the cell plays a crucial role in the action of arsenite coclastogenicity. One prediction from this finding is that in combined treatment, posttreatment with sodium arsenite should preferentially kill cancer cells.
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References
Cohen A, Barankiewicz J, Lederman JN, Gelfand EW (1983) Purine and pyrimidine metabolism in human T-lymphocytes: regulation of deoxyribonucleotide metabolism. J Biol Chem 258:12334–12340
Downes CS, Collins ARS, Johnson RT (1983) International workshop on inhibition of DNA repair. Mutat Res 112:75–83
Hancock R, Boulikas T (1982) Functional organization in the nucleus. Int Rev Cytol 79:165–214
Heartlein MW, Preston RJ (1985) The effect of 3-aminobenzamide on the frequency of X-ray- or neutron-induced chromosome aberrations in cycling or non-cycling human lymphocytes. Mutat Res 148:91–97
Jan KY, Wuu SW, Wen WN (1982) A simplified fluorescence plus Giemsa method for consistent differential staining of sister chromatids. Stain Technol 57:45–46
Jan KY, Huang RY, Lee TC (1986) Differential modes of action of sodium arsenite, 3-aminobenzamide, and caffeine on the enhancement of ethyl methanesulfonate clastogenicity. Cytogenet Cell Genet 41:202–208
Kalweit S, Obe G (1984) Liquid-holding experiments with human peripheral lymphocytes II. Experiments with trenimon and 1-β-d-arabinosylcytosine. Mutat Res 128:59–64
Kyriazis AP, Kyriazis AA, Yagoda A (1985) Enhanced therapeutic effect of cis-diamminedichloroplatinum (II) against nude mousegrown human pancreatic adenocarcinoma when combined with 1-β-d-arabinofuranosyl cytosine and caffeine. Cancer Res 45: 6083–6087
Lee TC, Huang RY, Jan KY (1985) Sodium arsenite enhances the cytotoxicity, clastogenicity, and 6-thioguanine-resistant mutagenicity of ultraviolet light in Chinese hamster ovary cells. Mutat Res 148:83–89
Lee TC, Lee KC, Tzeng YJ, Huang RY, Jan KY (1986a) Sodium arsenite potentiates the clastogenicity and mutagenicity of DNA crosslinking agents. Environ Mutagen 8:119–128
Lee TC, Wang-Wuu S, Huang RY, Lee KCC, Jan KY (1986b) Differential effects of pre- and posttreatment of sodium arsenite on the genotoxicity of methyl methanesulfonate in Chinese hamster ovary cells. Cancer Res 46:1854–1857
Lee TC, Tzeng SF, Chang WJ, Lin YC, Jan KY (1986c) Post-treatments with sodium arsenite during G2 enhance the frequency of chromosomal aberrations induced by S-dependent clastogens. Mutat Res 163:263–269
MacRae WD, MacKinnon EA, Stich HF (1979) The fate of UV-induced lesions affecting SCEs, chromosome aberrations and survival of CHD cells arrested by deprivation of arginine. Chromosoma 72:15–22
Snyder RD (1984) The role of deoxynucleoside triphosphate pools in the inhibition of DNA-excision repair and replication in human cells by hydroxyurea. Mutat Res 131:163–172
Snyder RD, Regan JD (1982) Differential responses of log and stationary phase human fibroblasts to inhibition of DNA repair by aphidicolin. Biochim Biophys Acta 697:229–234
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Huang, R.Y., Jan, K.Y. & Lee, T.C. Posttreatment with sodium arsenite is coclastogenic in log phase but not in stationary phase. Hum Genet 75, 159–162 (1987). https://doi.org/10.1007/BF00591079
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DOI: https://doi.org/10.1007/BF00591079