Abstract
In the present study, we examined the effects of guanine nucleotides on vasopressin-induced osmotic water flow and sodium transport in the 14-h preincubated frog bladder. We also examined the effects of the adenylate cyclase-cyclic AMP and cyclic AMP-dependent protein kinase system in the bladder's epithelial cells.
Gpp(NH)p significantly enhanced vasopressin-induced water flow while it did not affect cyclic AMP-induced water flow. However, Gpp(NH)p did not enhance the vasopressin-induced increment of sodium transport across the frog bladder.
The adenylate cyclase activity of the crude homogenate was enhanced by vasopressin, Gpp(NH)p and NaF. The effects of Gpp(NH)p and vasopressin, at their maximum doses, on the enzyme activities were additive, while other combinations were not. Specific Gpp(NH)p binding sites were found in the pellet fraction after 2,400×g centrifugation.
No direct effect on the protein kinase activity was observed in the presence of 10−6 M nucleotides, such as GTP, GDP, GMP, CTP, UTP, ITP and Gpp(NH)p. Cyclic AMP stimulated the phosphorylation of discrete protein bands, however, Gpp(NH)p did not influence cyclic AMP-dependent protein phosphorylation of crude homogenate of the bladder's epithelial cells.
These results suggest the guanine nucleotides stimulate the vasopressin-induced osmotic water flow in frog bladder by enhancing the vasopressin-mediated adenylate cyclase activity, so that accumulated cyclic AMP might activate cyclic AMP-dependent protein kinase.
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Abbreviations
- GTP:
-
guanosine 5′-triphosphate
- GDP:
-
guanosine 5′-diphosphate
- GMP:
-
guanosine 5′-monophosphate
- Gpp(NH)p:
-
5′-guanylylimidodiphosphate (β-γ-imidoguanosine 5′-monophosphate)
- CTP:
-
cytidine 5′-triphosphate
- UTP:
-
uridine 5′-triphosphate
- ITP:
-
inosine 5′-triphosphate
References
Bentley PJ (1959) The effects of ionic changes on water transfer across the isolated urinary bladder of the toad,Bufo marinus. J Endocrinol 18:327–333
Bentley PJ (1960) The effects of vasopressin on the short-circuit current across the wall of the isolated bladder of the toad,Bufo marinus. J Endocrinol 21:161–170
Brinbaumer L, Yang PC (1974) Studies on receptor-mediated activation of adenylate cyclase. J Biol Chem 249:7848–7856
DeLange RJ, Kemp RG, Riley WD, Cooper RA, Krebs EG (1968) Activation of skeletal muscle phosphorylase kinase by adenosine triphosphate and adenosine 3′,5′-monophosphate. J Biol Chem 243:2200–2208
DeLorenzo RJ, Walton KG, Curran PF, Greengard P (1973) Regulation of phosphorylation of a specific protein in toad-bladder membrane by antidiuretic hormone and cyclic AMP, and its possible relationship to membrane permeability changes. Proc Natl Acad Sci USA 70:880–884
Dousa TP, Sands H, Hechter O (1972) Cyclic AMP-dependent reversible phosphorylation of renal medullary plasma membrane protein. Endocrinology 91:757–763
Dousa TP, Valtin H (1976) Cellular actions of vasopressin in the mammalian kidney. Kidney Int 10:46–63
Edwards RM, Jackson BA, Dousa TP (1980) Protein kinase activity in isolated tubules of rat renal medulla. Am J Physiol 238:F269–F278
Ferguson DR, Twite BR (1974) Effects of vasopressin on toad bladder membrane proteins. J Endocrinol 61:501–507
Frindt G, Burg MB (1972) Effect of vasopressin on sodium transport in renal cortical collecting tubules. Kidney Int 1:224–231
Gilman AG (1970) A protein binding assay for adenosine 3′,5′-cyclic monophosphate. Proc Natl Acad Sci USA 67:305–312
Imbert-Teboul M, Chabardes D, Montegut M, Clique A, Morel F (1978) Vasopressin-dependent adenylate cyclase activities in the rat kidney medulla. Endocrinology 102:1254–1264
Jard S, Bastide F (1970) A cyclic AMP-dependent protein kinase from frog bladder epithelial cells. Biochem Biophys Res Com 39:559–566
Krishna G, Harwood J, Barber AJ, Jamieson GA (1972) Requirement for guanosin triphosphate in the prostaglandin activation of adenylate cyclase of platelet membranes. J Biol Chem 247:2253–2254
Lipton P, Edelman IS (1971) Effects of aldosterone and vasopressin on electrolytes of toad bladder epithelial cells. Am J Physiol 221:733–741
Londos C, Salomon Y, Lin MC, Harwood JP, Schumann M, Orloff J, Rodbell M (1974) 5′-Guanylylimidodiphosphate, a potent activator of adenylate cyclase systems in eukaryotic cells. Proc Natl Acad Sci USA 71:3087–3090
Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Maffly RH, Edelman IS (1963) The coupling of the short-circuit current to metabolism in the urinary bladder of the toad. J Gen Physiol 46:732–754
Marumo F (1978) Stimulative effect of guanylylimidodiphosphate on the vasopressin-induced increment of osmotic water flow of the toad bladder. Life Science 23:907–912
Marumo F, Edelman IS (1971) Effects of Ca2+ and prostaglandin E1 on vasopressin activation of renal adenylate cyclase. J Clin Invest 50:1613–1620
Marumo F, Inatsuki B, Kikawada R (1973) The effect of maltose on the aldosterone activated sodium transport of the frog bladder. Experimentia 29:43–44
Marumo F, Kikawada R (1974) Effect of phospholipase C on the permeability of the toad bladder. Jpn Cir J 38:271–276
Marumo F, Mishina T (1978) Effects of ethacrynic acid and furosemide on the hormone-mediated adenylate cyclase activation of the hamster kidney. Endocrinol Jpn 25:423–430
Nakamura S, Fugiki S (1968) Comparative studies on the glucose oxidases ofAspergillus niger andPenicillium amagasakiens. J Biochem 63:51–58
Omachi RS, Robbie DE, Handler JS, Orloff J (1974) Effects of ADH and other agents on cyclic AMP accumulation in toad bladder epithelium. Am J Physiol 226:1152–1157
Orloff J, Handler JS (1965) Effect of prostagladin (PGE1) on the permeability response of toad bladder to vasopressin, theophylline and adenosin 3′,5′-monophosphate. Nature 205:397–398
Porter GA, Bogoroch R, Edelman IS (1964) On the mechanism of action of aldosterone on sodium transport: The role of RNA synthesis. Proc Natl Acad Sci USA 52:1326–1333
Rodbell M (1980) The role of hormone receptors and GTP-regulatory proteins in membrane transduction. Nature 284:17–22
Rodbell M, Lin MC, Salomon Y, Londos C, Harwood JP, Rendell M, Berman M (1974) The role of adenine and guanine nucleotides in the activity and response of adenylate cyclase systems to hormones. Acta Endocrinol 77, Suppl 191:11–37
Rudolph SA, Krueger BK (1979) Endogenous protein phosphorylation and dephosphorylation. Adv Cyclic Nucleotide Res 10:107–133
Salomon Y, Londos C, Rodbell M (1974) A highly sensitive adenylate cyclase assay. Analytical Biochem 58:541–548
Schlondorff D, Franki N (1980) Effect of vasopressin on cyclic AMP-dependent protein kinase in toad urinary bladder. Biochim Biophys Acta 628:1–12
Schwartz IL, Shlatz LJ, Kinne-Saffran E, Kinne R (1974) Target cell polarity and membrane phosphorylation in relation to the mechanism of action of antidiuretic hormone. Proc Natl Acad Sci USA 71:2595–2599
Shimada H, Marumo F (1980) Effects of guanine nucleotides on vasopressin-induced increment of water permeability of the toad bladder. Jpn J Nephrol 22:111–118
Skala JP, Knight BL (1975) Protein kinases in brown adipose tissue of developing rats. Biochim Biophys Acta 385:114–123
Ussing HH, Zerahn K (1951) Active transport of sodium as the source of electric current in the short-circuit isolated frog skin. Acta Phys Scand 23:110–127
Wolff J, Cook GH (1973) Activation of thyroid membrane adenylate cyclase by purine nucleotides. J Biol Chem 248:350–355
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Shimada, H., Mishina, T. & Marumo, F. Effects of guanine nucleotides on vasopressin-induced water flow and sodium transport of the frog bladder. Pflugers Arch. 397, 169–175 (1983). https://doi.org/10.1007/BF00584353
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DOI: https://doi.org/10.1007/BF00584353