Pflügers Archiv

, Volume 402, Issue 4, pp 353–359 | Cite as

Effect of various cations and anions on the action of tetrodotoxin and saxitoxin on frog myelinated nerve fibers

  • S. Grissmer
Excitable Tissues and Central Nervous Physiology

Abstract

The influence of Mg2+, La3+, NO 3 , and SCN on the equilibrium effect of tetrodotoxin (TTX) and saxitoxin (STX) on single myelinated nerve fibres of the frogRana esculenta was studied under voltage clamp conditions. Mg2+ and La3+ reduce the sodium permeability, shift the voltage dependence of the Na permeabilityPNa towards more positive potentials and reduce the effectiveness of TTX and STX. NO 3 and SCN reduce the sodium permeability too, but shift the voltage dependence ofPNa towards more negative potentials and increase the action of TTX and STX. In all experiments the change in effectiveness is larger for the divalent STX than for the monovalent TTX. It is concluded that changes of the external surface potential induced by Mg2+, La3+, NO 3 and SCN affect the TTX and STX binding to toxin receptors. The apparent potential change at the toxin receptor is only a fraction of the change ‘seen’ by the Na channel gates.

Key words

Frog node of Ranvier Voltage clamp TTX STX Surface charge 

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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • S. Grissmer
    • 1
  1. 1.I. Physiologisches InstitutUniversität des SaarlandesHomburg/SaarFederal Republic of Germany

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