Abstract
A genetic polymorphism of rabbit (Oryctolagus cuniculus) hemoglobin α chain is demonstrated by means of acid starch gel electrophoresis. The polymorphism is not detected by isoelectric focusing and may be based upon neutral for neutral amino acid substitutions in accordance with previous findings by means of amino acid sequencing. Segregation analysis was performed on 15 matings with 49 offspring and confirmed the initial genetic hypothesis of three common codominant alleles at an autosomal locus. The calculated gene frequencies in a random sample of 86 unrelated individuals areHBA*1=0.73,HBA*2=0.22, andHBA*3=0.05.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Altland, K., and Banzhoff, A. (1986). Separation by hybrid isoelectric focusing of normal human plasma transthyretin (prealbumin) and a variant with a methionine for valine substitution associated with familial amyloidotic polyneuropathy.Electrophoresis 7529.
Altland, K., Becher, P., and Banzhoff, A. (1987). Paraffin oil protected high resolution hybrid isoelectric focusing for the demonstration of substitutions of neutral amino acids in denatured proteins: The case of four human transthyretin (prealbumin) variants associated with familial amyloidotic polyneuropathy.Electrophoresis 8293.
Bricker, J., and Garrick, M. D. (1974). An isoleucine-valine substitution in the β-chain of rabbit hemoglobin.Biochim. Biophys. Acta 351437.
Caspers, M. L., Posey, Y., and Brown, R. K. (1977). Separator isoelectric focusing: An improved method of protein analysis and purification.Anal. Biochem. 79166.
Ferrand, N. (1989). Biochemical and genetic studies on rabbit hemoglobin. I. Electrophoretic polymorphism of the β-chain.Biochem. Genet. 27673.
Galizzi, A. (1970). The amino acid sequence of the β-chains of rabbit hemoglobin.Eur. J. Biochem. 1749.
Galizzi, A. (1971). Polymorphic alleles of the gene for the β chain of rabbit haemoglobin.Nature New Biol. 229142.
Garrick, M. D., Bricker, J., and Garrick, L. M. (1974). An electrophoretically silent polymorphism for the beta chains of rabbit hemoglobin and associated polyribosome patterns.Genetics 7699.
Hunter, T., and Munro, A. (1969). Allelic variants in the amino-acid sequence of the α-chain of rabbit hemoglobin.Nature 2231270.
McIlwaine, I., Rodbard, D., and Chrambach, A. (1973). Characterization by polyacrylamide gel electrophoresis of hemoglobins in rabbit embryo, fetus and adult.Anal. Biochem. 55521.
Righetti, P. G. (1983).Isoelectric Focusing: Theory, Methodology and Applications Elsevier, Amsterdam.
von Ehrenstein, G. (1966). Translational variation in the amino acid sequence of the α-chain of rabbit hemoglobin.Cold Spring Harbor Symp. Quant. Biol. 31705.
Zaragoza, P., Arana, A., Zarazaga, I., and Amorena, B. (1987). Blood biochemical polymorphisms in rabbits presently bred in Spain: Genetic variation and distances amongst populations.Aust. J. Biol. Sci. 40275.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ferrand, N. Biochemical and genetic studies on rabbit hemoglobin. II. Electrophoretic polymorphism of theα-chain. Biochem Genet 28, 117–122 (1990). https://doi.org/10.1007/BF00561331
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00561331