Abstract
Chromosomal DNA measurements represent the most accurate methodology for the quantitative analysis of human karyotype. A modified FACS 440 signal collection is described here. The FSC signal voltage coming from the optical bench has been split in two equal parts by a T-connector placed at the preamplifier entrance. The side scatter channel that is not employed during chromosome analysis has been used for the half-FSC signal. This modification allowed the contemporary use of FSC signal both for gating and analysis leading to a better discrimination of background, and therefore to a more accurate definition of sorting windows.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
HarrisP, BoydE, YoungBD and Ferguson-SmithMA (1986) Determination of the DNA content of human chromosomes by flow cytometry. Cytogenet. Cell Genet. 41: 14.
YoungBD, Ferguson-SmithMA, SillarR and BoydE (1981) High-resolution analysis of human peripheral lymphocyte chromosomes by flow cytometry. Proc. Natl. Acad. Sci. USA 78(12): 7727.
HarrisP, CookeA, BoydE, YoungBD and Ferguson-SmithMA (1987) The potential of family flow karyotyping for the detection of chromosome abnormalities. Hum. Genet. 76: 129.
AtenJA, BuysCHCM, van derVeenAY, MesaJR, YuLC, GrayJW, OsingaJ and StapJ (1897) Stabilization of chromosomes by DNA intercalators for flow karyotyping and identification by banding of isolated chromosomes. Histochem. 87: 359.
Van denEnghG, TraskB, CramS and BartholdyM (1984) Preparation of chromosomes suspensions for flow cytometry. Cytometry 5: 108.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Vitale, M., Zamai, L., Antonucci, A. et al. Technical improvements for flow karyotyping by standard FACS 440 flow cytometer. Cytotechnology 5, 195–199 (1991). https://doi.org/10.1007/BF00556289
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00556289