Summary
When rat peritoneal macrophages were stimulated with concanavalin A (Con A), phosphatidylinositol (PI) turnover was rapidly induced and concomitantly N-acetylglucosaminidase was released. These two reactions were markedly inhibited by prostaglandin E1, which elevated the cellular cyclic AMP levels. This effect was mimicked by dibutyryl cyclic AMP. These results suggest that the elevation of the cellular cyclic AMP levels might play a role in the inhibition of the early stages of Con A-induced activation of macrophages through the inhibition of PI turnover.
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References
Weissmann G, Spilberg I, Krakauer K (1969) Arthritis induced in rabbits by lysates of granulocyte lysosomes. Arthritis Rheum 12:103–116
Weissmann G (1972) Lysosomal mechanisms of tissue injury in arthritis. N Engl J Med 286:141–147
Allen JM, Cook GMW, Poole AR (1971) Action of concanavalin A on the attachment stage of phagocytosis by macrophages. Exp Cell Res 68:466–471
Smith CW, Goldman AS (1971) Macrophages from human colostrum: Multinucleated giant cell formation by phytohemagglutinin and concanavalin A. Exp Cell Res 66:317–320
Lutton JD (1973) The effect of phagocytosis and spreading on macrophage surface receptors for concanavalin A. J Cell Biol 56:611–617
Goldman R, Raz A (1975) Concanavalin A and the in vitro induction in macrophages of vacuolation and lysosomal enzyme synthesis. Exp Cell Res 96:393–405
Romeo D, Zabucchi G, Rossi F (1973) Reversible metabolic stimulation of polymorphonuclear leukocytes and macrophages by concanavalin A. Nature 243:111–112
Petty HR, Ware BR (1979) Macrophage response to concanavalin A: Effect of surface cross-linking on the electrophoretic mobility distribution. Proc Natl Acad Sci USA 76:2278–2282
Michell RH (1975) Inositol phospholipids and cell surface receptor function. Biochim Biophys Acta 415:81–147
Rubin RP, Sink LE, Freer RJ (1981) On the relationship between formylmethionyl-leucyl-phenylalanine stimulation of arachidonyl phosphatidylinositol turnover and lysosomal enzyme secretion by rabbit neutrophils. Mol Pharmacol 19:31–37
Weissmann G, Dukor P, Zurier RB (1971) Effect of cyclic AMP on release of lysosomal enzymes from phagocytes. Nature 231:131–135
Skipski VP, Peterson RF, Barclay M (1964) Quantitative analysis of phospholipids by thin-layer chromatography. Biochem J 90:374–378
Wacker WEC, Ulmer DD, Vallee BL (1956) Metalloenzymes and myocardial infarction. II. Malic and lactic dehydrogenase activities and zinc concentration in serum. N Engl J Med 255:449–456
Steiner AL, Pagliara AS, Chase LR, Kipnis DM (1972) Radioimmunoassay for cyclic nucleotides. II. Adenosin 3′,5′-monophosphate and guanosine 3′,5′-monophosphate in mammalian tissues and body fluids. J Biol Chem 247: 1114–1120
Wightman PD, Dahlgren ME, Hall JC, Davies P, Bonney RJ (1981) Identification and characterization of a phospholipase C activity in resisdent mouse peritoneal macrophages. Biochem J 197:523–526
Ögmundsdottir HM, Weir DM (1979) Stimulation of phosphatidylinositol turnover in the macrophage plasma membrane: A possible mechanism for signal transmission. Immunology 37:689–696
Kawahara T, Takai Y, Minakuchi R, Sano K, Nishizuka Y (1980) Possible involvement of Ca2+-activated, phospholipid-dependent protein kinase in platelet activation. J Biochem 88:913–916
Cockcroft S, Gomperts BD (1979) Evidence for a role of phosphatidylinositol turnover in stimulus-secretion coupling. Studies with rat peritoneal mast cells. Biochem J 178: 681–687
Kennerly DA, Secosan CJ, Parker CW, Sullivan TJ (1979) Modulation of stimulated phospholipid metabolism in mast cells by pharmacologic agents that increase 3′,5′-adenosine monophosphate levels. J Immunol 123:1519–1524
Billah MM, Lapetina EG, Cuatrecasas P (1979) Phosphatidyl-inositol-specific phospholipase C of platelets: Association with 1,2-diacylglycerol-kinase and inhibition by cyclic AMP. Biochem Biophys Res Commun 90:92–98
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Matsubara, T., Fujii, M., Ishikawa, H. et al. Inhibition of concanavalin A-induced phosphatidylinositol turnover and lysosomal enzyme release by cyclic AMP-elevating agents in rat peritoneal macrophages. Rheumatol Int 3, 29–33 (1983). https://doi.org/10.1007/BF00541229
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DOI: https://doi.org/10.1007/BF00541229