Summary
The interaction between presynaptic, release-inhibiting α 2-adrenoceptors and opioid receptors was studied in slices of the parieto-occipital cortex of rabbits. The slices were preincubated with 3H-noradrenaline and then superfused with 3H-noradrenaline-free medium and stimulated electrically (3 or 7 Hz, 2 or 5 V/cm voltage drop between the electrodes). Clonidine and ethylketocyclazocine (EK) depressed, whereas yohimbine increased the electrically evoked overflow of tritium. When clonidine was administered first and retained in the medium for the rest of the experiment, the overflow-inhibiting effect of EK was reduced. When yohimbine was administered first and kept for the rest of the experiment, the effect of EK was enhanced. When, finally, EK was adminstered first and clonidine as the second drug, the overflow-inhibiting effect of clonidine was attenuated. The changes in the effect of EK (by clonidine or yohimbine) and clonidine (by EK) were not due to the changes in release per se produced by the drugs that were given first. Naloxone shifted the concentration-response curve of EK to the right; the dissociation constant of the naloxone-receptor complex, calculated from the shift, was 13 nmol/l. It is concluded that there is an interaction between presynaptic α 2-adrenoceptors and opioid к-receptors, either at the level of the receptors themselves or of the post-receptor reaction chains. Activation of one kind of receptor blunts the inhibition of release produced by activation of the other kind of receptor.
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References
Endo T, Starke K, Bangerter A, Taube HD (1977) Presynaptic receptor systems on the noradrenergic neurones of the rabbit pulmonary artery. Naunyn-Schmiedeberg's Arch Pharmacol 296:229–247
Furchgott RF (1972) The classification of adrenoceptors (adrenergic receptors). An evaluation from the standpoint of receptor theory. In: Blaschko H, Muscholl E (eds) Catecholamines. Handbook of experimental pharmacology, vol XXXIII. Springer, Berlin Heidelberg New York, pp 283–335
Garcia-Sevilla JA, Dubocovich ML, Langer SZ (1985) Interaction between presynaptic facilitatory angiotensin II receptors and inhibitory muscarinic cholinoceptors on 3H-noradrenaline release in the rabbit heart. Naunyn-Schmiedeberg's Arch Pharmacol 330:9–15
Hedqvist P (1974) Prostaglandin action on noradrenaline release and mechanical responses in the stimulated guinea pig vas deferens. Acta Physiol Scand 90:86–93
Hölting T, Starke K (1986) Receptor protection experiments confirm the identity of presynaptic α 2-autoreceptors. Naunyn-Schmiedeberg's Arch Pharmacol 333:262–270
Illes P, Dörge L (1985) Mechanism of α 2-adrenergic inhibition of neuroeffector transmission in the mouse vas deferens. Naunyn-Schmiedeberg's Arch Pharmacol 328:241–247
Jackisch R, Geppert M, Illes P (1986) Characterization of opioid receptors modulating noradrenaline release in the hippocampus of the rabbit. J Neurochem 46:1802–1810
Kosterlitz HW (1985) Opioid peptides and their receptors. Proc R Soc Lond B 225:27–40
Loiacono RE, Rand MJ, Story DF (1985) Interaction between the inhibitory action of acetylcholine and the α-adrenoceptor autoinhibitory feedback system on release of [3H]-noradrenaline from rat atria and rabbit ear artery. Br J Pharmacol 84:697–705
Majewski H, Rand MJ (1981) An interaction between prejunctional α-adrenoceptors and prejunctional β-adrenoceptors. Eur J Pharmacol 69:493–498
Massingham R, Dubocovich ML, Langer SZ (1980) The role of presynaptic receptors in the cardiovascular actions of N,N-din-propyldopamine in the cat and dog. Naunyn-Schmiedeberg's Arch Pharmacol 314:17–28
North RA (1986) Opioid receptor types and membrane ion channels. Trends in Neurosciences 9:114–117
Ramme D, Illes P, Späth L, Starke K (1986) Blockade of α 2-adrenoceptors permits the operation of otherwise silent opioid к-receptors at the sympathetic axons of rabbit jejunal arteries. Naunyn-Schmiedeberg's Arch Pharmacol (in press)
Reichenbacher D, Reimann W, Starke K (1982) α-adrenoceptormediated inhibition of noradrenaline release in rabbit brain cortex slices. Nauyn-Schmiedeberg's Arch Pharmacol 319:71–77
Schoffelmeer ANM, Putters J, Mulder AH (1986) Activation of presynaptic α 2-adrenoceptors attenuates the inhibitory effect of μ-opioid receptor agonists on noradrenaline release from brain slices. Naunyn-Schmiedeberg's Arch Pharmacol (in press)
Starke K, Schümann HJ (1972) Interaction of angiotensin, phenoxybenzamine and propranolol on noradrenaline release during sympathetic nerve stimulation. Eur J Pharmacol 18:27–30
Vizi ES, Ono K, Adam-Vizi V, Duncalf D, Földes FF (1984) Presynaptic inhibitory effect of Met-enkephalin on [14C]acetulcholine release from the myenteric plexus and it interaction with muscarinic negative feedback inhibition. J Pharmacol Exp Ther 230:493–499
Weitzell R, Illes P, Starke K (1984) Inhibition via opioid μ- and δ-receptors of vagal transmission in rabbit isolated heart. Naunyn-Schmiedeberg's Arch Pharmacol 328:186–190
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Limberger, N., Späth, L., Hölting, T. et al. Mutual interaction between presynaptic α 2-adrenoceptors and opioid к-receptors at the noradrenergic axons of rabbit brain cortex. Naunyn-Schmiedeberg's Arch. Pharmacol. 334, 166–171 (1986). https://doi.org/10.1007/BF00505817
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DOI: https://doi.org/10.1007/BF00505817