Summary
In frog sartorius muscles, reduction of [Ca++]0 enhanced the depolarization of the end-plate membrane caused by nicotine while elevation of [Ca++]0 reduced the nicotine-induced depolarization. A quantitative study of the effects of [Ca++]0 on the nicotine-induced depolarization indicated that the interaction between calcium and nicotine was noncompetitive. Reduction of the chloride concentration or acidification of the Ringer's solution enhanced the depolarization caused by nicotine (0.015–0.10 mM) and antagonized the influence of elevated [Ca++]0. The enhanced sensitivity of muscles to nicotine at low pH and in Cl−-free solutions was probably due to the increased effective resistance of the muscle membrane caused by these conditions. The effects of variation of [Ca++]0 appeared to be independent of changes in the membrane resistance.
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This study was supported by a grant from the AMA-ERF
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Henderson, E.G., Reynolds, L. Modification of the nicotine-induced depolarization in striated muscle by altered [H+]0, [Ca++]0 and [Cl−]0 . Naunyn-Schmiedeberg's Arch. Pharmacol. 278, 81–90 (1973). https://doi.org/10.1007/BF00501865
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DOI: https://doi.org/10.1007/BF00501865