Summary
The effect of histamine, pentagastrin and theophylline on the cyclic AMP level was studied in minced gastric tissue and isolated gastric mucosa of the guinea pig:
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1.
In the presence of high concentrations of minced tissue in the incubate theophylline (10−3 M), but not histamine (10−3 M), alone induced a significant elevation of the content of cyclic AMP.
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2.
In contrast, in the presence of low amounts of isolated gastric mucosa histamine (6×10−4 M), but not theophylline (10−3 M), alone caused an elevation of the content of cyclic AMP.
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3.
The combination of histamine (6×10−4 M or 10−3 M) and theophylline (10−3 M) caused a marked elevation in the level of cyclic AMP. However, the magnitude of this elevation was inversely related to the amount of tissue in the incubate.
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4.
The increase of the content of cyclic AMP, induced by the combination of histamine and theophylline in the isolated mucosa, was inhibited by the histamine H2-receptor antagonist burimamide, but not by the histamine H1-receptor blocking agent diphenydramine.
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5.
Pentagastrin alone or in combination with theophylline did not significantly change the level of cyclic AMP in the isolated tissue.
The results demonstrate that the histamine-induced increase of cyclic AMP in the gastric mucosa is mediated via histamine H2-receptors, the stimulation of which is also known to increase the secretion of gastric acid. Accordingly, cyclic AMP may be the intracellular mediator of the histamine-induced secretion of gastric acid. In contrast, the pentagastrin-induced secretion of gastric acid in the guinea pig does not seem to be attributable to an increase in the level of cyclic AMP.
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Karppanen, H.O., Neuvonen, P.J., Bieck, P.R. et al. Effect of histamine, pentagastrin and theophylline on the production of cyclic AMP in isolated gastric tissue of the guinea pig. Naunyn-Schmiedeberg's Arch. Pharmacol. 284, 15–23 (1974). https://doi.org/10.1007/BF00499969
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DOI: https://doi.org/10.1007/BF00499969