Abstract
The relationship between the rapidly (component I) and slowly (components II) migrating components of placental alkaline phosphatase has been studied. Storage of components II resulted in conversion into component I with a parallel increase of activity. The conversion rate increased with temperature. The fastest of the slow-moving components (component IIα) was less stable than the very slow-migrating ones (components IIβ). Component IIα was not identifiable after 25 days at 37 C, while some of the IIβ components were still present after a period of 1 year. No conversion of component I into II was observed. Data from this study suggest that the apparent thermostability of placental alkaline phosphatase results partly from the increase in activity after the conversion of components II into component I.
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This work was supported in part by U.S. Public Health Service Grant HD02552. K.H. is a Career Scientist of the Health Research Council of the City of New York (I-513).
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Beratis, N.G., Seegers, W. & Hirschhorn, K. Properties of placental alkaline phosphatase. II. Interactions of fast- and slow-migrating components. Biochem Genet 5, 367–377 (1971). https://doi.org/10.1007/BF00485862
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DOI: https://doi.org/10.1007/BF00485862