Abstract
Chromatium vinosum cells form a vesicular type intracytoplasmic membrane system during phototrophic growth on thiosulfate.—An enzyme protein transferring electrons from thiosulfate to cytochromes of type c was enriched from S-144. The colorless thiosulfate: cytochrome c oxidoreductase was characterized by a molecular weight of 36,000 (after dodecylsulfate treatment) and 35,000 (by gel filtration). Isoelectric focusing revealed a pI range of 4.4 to 4.7. Apparent K m values for the cytochromes tested were in the μM range. — The endogenous electron acceptor compound, isolated from the chromatophore fraction P-144, was found to be a membrane-bound cytochrome c-552. The homogeneous cytochrome protein had an average pI value of 4.65 and a molecular weight of 71,500 determined by gel filtration. By dodecylsulfate electrophoresis it was cleaved into two proteins representing particle weights of 45,000 and 20,000.
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Abbreviations
- HiPIP:
-
high potential nonheme iron protein
- IEF:
-
isoelectric focusing
- SDS:
-
dodecylsulfate, sodium salt
- Temed:
-
N,N,N′,N′-tetramethylethylenediamine
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Schmitt, W., Schleifer, G. & Knobloch, K. The enzymatic system thiosulfate: Cytochrome c oxidoreductase from photolithoautotrophically grown Chromatium vinosum . Arch. Microbiol. 130, 334–338 (1981). https://doi.org/10.1007/BF00425951
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DOI: https://doi.org/10.1007/BF00425951