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Presence of a trimethylamine: HS-coenzyme M methyltransferase in Methanosarcina barkeri

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Abstract

A trimethylamine:2-mercaptoethanesulfonate (HS-coenzyme M) methyltransferase has been shown to be present in trimethylamine-grown cells but not in methanol-grown cells of Methanosarcina barkeri. The transfer of one methyl group was catalyzed by this enzyme so that dimethylamine and methyl-S-coenzyme M were the products. Enzyme activity required the presence of ATP and preincubation of the protein solution under H2. Fifty percent of the maximum activity was obtained under N2 in the presence of NAD(P)H plus dithioerythritol.

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Abbreviations

HS-coenzyme M:

2-mercaptoethanesulfonic acid

methyl-S-coenzyme M:

2-(methylthio)ethanesulfonic acid

TES:

N-tris (hydroxymethyl)-methyl-2-aminoethanesulfonic acid

DTE:

1,4-dithioerythritol

BrES:

2-bromoethanesulfonic acid

DTT:

1,4-dithiothreotol

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Authors and Affiliations

  1. Institut für Mikrobiologie, Universität Göttingen, D-3400, Göttingen, Germany

    Evelyn Naumann, Karoline Fahlbusch & Gerhard Gottschalk

Authors
  1. Evelyn Naumann
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  2. Karoline Fahlbusch
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  3. Gerhard Gottschalk
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Naumann, E., Fahlbusch, K. & Gottschalk, G. Presence of a trimethylamine: HS-coenzyme M methyltransferase in Methanosarcina barkeri . Arch. Microbiol. 138, 79–83 (1984). https://doi.org/10.1007/BF00425412

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  • DOI: https://doi.org/10.1007/BF00425412

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