Abstract
The quantitative changes of double-stranded RNA components of nuclear ribonucleo-protein particles containing pre-mRNA was investigated in the course of incubation of particles at 37 ° C. The incubation of purified nuclear particles revealed the fragmentation of long double-stranded RNA sequences into shorter stretches. The presence of nuclear sap in the incubation mixture resulted in degradation of the double-stranded RNAs into acid soluble products. Autodegradation and/or ribonuclease treatment of nuclear RNP particles is accompanied by quantitative changes in the minor protein constituents of informofer.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ryskov, A. P., Farashyan, V. R., and Georgiev, G. P., Biochem. Biophys. Acta 262, 568 (1972).
Jelinek, W. and Darnell, J. E., Proc. Nat. Acad. Sci. U.S.A. 69, 2537 (1972).
Georgiev, G. P., Varshavsky, A. J., Ryskov, A. P., and Church, R. B., Cold Spring Harbor Symp. Quant. Biol. 38, 869 (1973).
Favre, A., Bentazzoni, U., Renns, A., and Bloemendal, H., Biochem. Biophys. Res. Comm. 56, 273 (1974).
Favre, A., Morel, C., and Scherrer, K., Eur. J. Biochem. 57, 147 (1975).
Holder, J. W. and Lingrel, J. B., Biochemistry 14, 4209 (1975).
Molnár, J., Besson, J., and Samarina, O. P., Mol. Biol. Reports 2, 11 (1975).
Samarina, O. P., Bajszár, G., and Molnár, J.: in E. J. Hidvégi, J. Sümegi and P. Venetianer (eds.), Biochemistry of Cell Nucleus. Mechanism and Regulation of Gene Expression. Vol. 33, Akadémiai Kiadó, Budapest, 1975, pp. 51–56.
Borisova, O. F., Molnár, J., Samarina, O. P., and Tsaregorodtsev, V. I., Mol. Biol. U.S.S.R., in press.
Scherrer, K.: in E. Diczfalusy (ed.), Protein Synthesis in Reproductive Tissue, Bogtrykkereiet Forum, Copenhagen, 1973, pp. 95–129.
Samarina, O. P., Lukanidin, E. M., Molnár, J., and Georgiev, G. P., J. Mol. Biol. 33, 251 (1968).
Ryskov, A. P., Saunders, G. F., Farashyan, V. R., and Georgiev, G. P., Biochem. Biophys. Acta 312, 152 (1975).
Samarina, O. P., Aitkozhina, N. A., and Besson, J., Mol. Biol. Reports 1, 193 (1973).
Weber, K. and Osborn, M., J. Biol. Chem. 244, 4406 (1969).
Molnár, J. and Samarina, O. P., mol. Biol. Reports 2, 1 (1975).
Molnár, J. and Komáromy, L., Acta Biochim. Biophys. Acad. Sci. Hung. 9, 73 (1974).
Birge, C. N. and Schlessinger, D., Federation Proc. 33, 1275 (1974).
Ryskov, A. P., Tokarskaya, O. V., and Georgiev, G. P., Mol. Biol. Reports 2, 353 (1976).
Sellwood, S. M., Riches, P. G., Harrap, K. R., Rickwood, D., and MacGillivary, A. J., Eur. J. Biochem. 52, 561 (1975).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Molnar, J., Samarina, O.P. Autodegradation of pre-mRNA containing nuclear ribo-nucleoprotein particles. The effect of autodegradation on the double-stranded RNA sequences and on the protein composition of particles. Mol Biol Rep 3, 195–202 (1976). https://doi.org/10.1007/BF00423235
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00423235