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Electron pathways from H2 to nitrate in Paracoccus denitrificans. Effects of inhibitors of the UQ-cytochrome b region

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Abstract

Electron flow from hydrogenase to nitrate reductase, in membranes of Paracoccus denitrificans cells grown autotrophically with nitrate as terminal electron acceptor, is inhibited by 2,5-dibromothymoquinone (DBMIB), 2,4-dinitrophenylether of iodonitrothymol (DNP-INT), 2-n-heptyl-4-hydroxy-quinoline-N-oxide (HQNO), and trifluraline, but is insensitive to antimycin A. A scheme of anaerobic electron flow from H2 to nitrate is proposed with inhibition sites for various compounds known to act in the ubiquinone-cytochrome b region.

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Abbreviations

DBMIB:

2,5-dibromothymoquinone

DNP-INT:

2,4-dinitrophenylether of iodonitrothymol

HQNO:

2-n-heptyl-4-hydroxyquinoline-N-oxide

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Authors and Affiliations

  1. Laboratoire de Biochimie (CNRS ER no 235, INSERM U. 191), Département de Recherche Fondamentale, Centre d'Etudes Nucléaires, 85X, F-38041, Grenoble cedex, France

    Michèle-F. Henry & Paulette M. Vignais

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  1. Michèle-F. Henry
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  2. Paulette M. Vignais
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Henry, MF., Vignais, P.M. Electron pathways from H2 to nitrate in Paracoccus denitrificans. Effects of inhibitors of the UQ-cytochrome b region. Arch. Microbiol. 136, 64–68 (1983). https://doi.org/10.1007/BF00415612

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  • DOI: https://doi.org/10.1007/BF00415612

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