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Growth inhibition of experimental glioma grafts by monoclonal antibody treatment

  • Original Papers
  • Experimental Oncology
  • Published:
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Summary

The effects of 14AC1 monoclonal antibody (McAb) on 79FR-G-41 rat glioma cells in vitro, on the formation of metastases in lung by antibody coated glioma cells, and on the growth of glioma grafts in BALB/c-nu/nu mice were investigated.

The 14AC1 antibodies — isotyped as IgG2a — were obtained from a hybridoma clone established after fusion of X63-Ag8.653 myeloma cells and spleen cells of BALB/c mice hyperimmunized with 79FR-G-41 glioma cells. Antibody treatment of glioma cells in vitro caused evident cell surface alterations and pronounced growth depression of most cells. However, a few tumor cells remained unchanged in morphology and continued to proliferate. Moreover, 14AC1 antibodies drastically reduced lung metastasis by pretreated and i.v. delivered glioma cells. Additionally, 14AC1 antibodies suppressed the growth of transplanted rat gliomas in nude mice as evidenced by a longer latency period and a smaller volume of glioma grafts in treated than in control tumor bearers. Nevertheless, glioma grafts showed accelerated growth after termination of antibody treatment.

Further experimental investigation is required in order to identify the precise mechanisms of the effects of McAbs on tumor cells in vitro and in vivo.

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Authors and Affiliations

  1. Institute of Pathology, Department of Clinical and Experimental Neuro-Oncology, Clinicum Bogenhausen, Teaching Hospital of the Technical University of Munich, D-8000, Munich, Federal Republic of Germany

    D. Stavrou & E. Keiditsch

  2. Department of Functional Pathology, Institute of Animal Pathology, University of Munich, D-8000, Munich, Federal Republic of Germany

    D. Stavrou, W. Mellert & U. Mellert

  3. Institute of Neuropathology, University of Munich, D-8000, Munich, Federal Republic of Germany

    K. Bise & P. Mehraein

Authors
  1. D. Stavrou
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  2. W. Mellert
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  3. U. Mellert
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  4. E. Keiditsch
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  5. K. Bise
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  6. P. Mehraein
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Stavrou, D., Mellert, W., Mellert, U. et al. Growth inhibition of experimental glioma grafts by monoclonal antibody treatment. J Cancer Res Clin Oncol 112, 111–118 (1986). https://doi.org/10.1007/BF00404392

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  • DOI: https://doi.org/10.1007/BF00404392

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