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Effect of growth condition on enzymes of the citric acid cycle and the glyoxylate cycle in the photosynthetic bacterium Rhodopseudomonas palustris

  • Biochemistry
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Abstract

The enzymes of the citric acid and glyoxylate cycles as well as RuBPFootnote 1 carboxylase were measured in cell-free extracts from Rhodopseudomonas palustris after growth under chemoheterotrophic, photoheterotrophic and photolithotrophic conditions. Although the citric acid cycle was found to be complete under all growth conditions, significant differences in certain enzyme activities occurred as a function of the different energy sources applied. The glyoxylate cycle also was complete under all growth conditions with highest isocitrate lyase activity seen after photoheterotrophic growth on acetate. Photo- and chemoheterotrophic growth on malate reduced the isocitrate lyase. The activity was not repressed further by photolithotrophic growth on thiosulfate. RuBP carboxylase activity, present under photolithotrophic conditions, was repressed by chemoheterotrophic growth but was not decreased by the presence of organic substrates during photoheterotrophic growth.

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Notes

  1. Abbreviations: EDTA, ethylenediamine tetraacetic acid; GSH, reduced glutathione; RuBP, ribulose bisphosphate.

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Author notes

  1. K. Knobloch

    Present address: Institut für Botanik u. Pharmazeutische Biologie, Universität Erlangen-Nürnberg, Schlossgarten 4, D-8520, Erlangen, Germany

Authors and Affiliations

  1. Biology Department, University of Colorado, 80907, Colorado Springs, Colorado, USA

    J. H. Eley

  2. T. H. Morgan School of Biological Sciences, University of Kentucky, 40506, Lexington, Kentucky, USA

    K. Knobloch & T. -W. Han

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Eley, J.H., Knobloch, K. & Han, T.W. Effect of growth condition on enzymes of the citric acid cycle and the glyoxylate cycle in the photosynthetic bacterium Rhodopseudomonas palustris . Antonie van Leeuwenhoek 45, 521–529 (1979). https://doi.org/10.1007/BF00403652

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