Abstract
In Drosophila, temperature shocks lead to the activation of definite puffs and to the appearance of definite new polypeptides. The effects of deletions and triplications of region 93D, the site of one of the largest inducible puffs, on the induced pattern of polypeptides has been studied. A direct correlation between the dose of this chromosomal region and the relative amount of the major inducible polypeptide (about 72,000 MW) has been observed. Uninduced embryos show a low basal level of synthesis of a 72,000 MW polypeptide and this synthesis sharply increases after temperature shocks. Out of a cross which segregates zygotes completely lacking the inducible site of region 93D, embryos were found which show no increased synthesis of the 72,000 MW polypeptide after temperature shocks. The inducible 72,000 MW polypeptide is distinctly larger than the larger subunit (about 66,000 MW) of glutamine synthetase 1, which is also induced by temperature shocks and whose structural gene was shown to map also in this region. Possible explanations are discussed.
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Scalenghe, F., Ritossa, F. The puff inducible in region 93D is responsible for the synthesis of the major “heat-shock” polypeptide in Drosophila melanogaster . Chromosoma 63, 317–327 (1977). https://doi.org/10.1007/BF00399494
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DOI: https://doi.org/10.1007/BF00399494