Abstract
A new, naturally occurring cytokinin was isolated from a cell culture of anise (Pimpinella anisum L.). Its structure was determined as 6-benzylaminopurine riboside by the use of gas chromatography and mass spectrometry. Quantitation of this new plant hormone was accomplished by single-ion-monitoring measurements.
Similar content being viewed by others
Abbreviations
- BAR:
-
6-benzylaminopurine riboside
References
Biddington, N.L., Thomas, T.H. (1973) A modified Amaranthus betacyanin bioassay for the rapid determination of cytokinins in plant cell extracts. Planta 111, 183–186
Chaves das Neves, H.J., Pais, M.S.S. (1980) A new cytokinin from the fruits of Zantedeschia aethiopica. Tetrahedron Lett. 21, 4387–4390
Ernst, D., Schäfer, W., Oesterhelt, D. (1983) Isolation and quantitation of isopentenyladenosine in an anise cell culture by single-ion monitoring, radioimmunoassay and bioassay. Planta 159, 216–221
Gilbert, M.L., Thompson, J.E., Dumbroff, E.B. (1980) Delayed cotyledon senescence following treatment with a cytokinin: an effect at the level of membranes. Can. J. Bot. 58, 1797–1803
Hashizume, T., Sugiyama, T., Imura, M., Cory, H.T., Scott, M.F., McCloskey, J.A. (1979) Determination of cytokinins by mass spectrometry based on stable isotope diluting. Anal. Biochem. 92, 111–122
Horgan, R., Hewett, E.W., Horgan, J.M., Purse, J., Wareing, P.F. (1975) A new cytokinin from Populus robusta. Phytochemistry 14, 1005–1008
Huber, J., Constabel, F., Gamborg, O.L. (1978) A cell counting procedure applied to embryogenesis in cell suspension cultures. Plant Sci. Lett. 12, 209–215
Letham, D.S. (1978) Cytokinins. In: Phytohormones and related compounds: a comprehensive treatise, vol. 1, pp. 205–251, Letham, D.S., Goodwin, P.B., Higgins, T.J.V., eds. Elsevier/North-Holland Biomedical Press, Amsterdam Oxford New York
Mackenzie, I.A., Konar, A., Street, H.E. (1972) Cytokinins and growth of cultured sycamore cells. New Phytol. 71, 633–638
Weiler, E.W., Spanier, K. (1981) Phytohormones in the formation of crown gall tumors. Planta 153, 326–337
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ernst, D., Schäfer, W. & Oesterhelt, D. Isolation and identification of a new, naturally occurring cytokinin (6-benzylaminopurineriboside) from an anise cell culture (Pimpinella anisum L.). Planta 159, 222–225 (1983). https://doi.org/10.1007/BF00397528
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00397528