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Isolation of heavy metal binding proteins from marine vertebrates

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Abstract

Cadmium binding proteins have been isolated from liver homogenates of marine vertebrates by ultracentri fugation and gel filtration. Liver samples of the Atlantic grey seal Halichoerus grypus and the Pacific fur seal Callorhinus ursinus contain measurable quantities of cadmium binding protein. The copper rock fish Sebastodes caurinus showed an increase in hepatic cadmium binding protein on administration of CdCl2, in agreement with the known inducible nature of the protein isolated from terrestrial animals. The apparent molecular weights of the isolated proteins were 9000 for the grey seal, 10,000 for the fur seal, and 11,000 for the copper rock fish, as determined by gel filtration.

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Authors and Affiliations

  1. Institute of Oceanography, University of British Columbia, Vancouver, British Columbia, Canada

    K. W. Olafson

  2. Pacific Environment Institute, West Vancouver, British Columbia, Canada

    J. A. J. Thompson

Authors
  1. K. W. Olafson
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  2. J. A. J. Thompson
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Communicated by T.R. Parsons, Vancouver

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Olafson, K.W., Thompson, J.A.J. Isolation of heavy metal binding proteins from marine vertebrates. Mar. Biol. 28, 83–86 (1974). https://doi.org/10.1007/BF00396298

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  • DOI: https://doi.org/10.1007/BF00396298

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