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Induction and complementation of lysine auxotrophs in Saccharomyces

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Abstract

Four chemical agents, EMSFootnote 1, MNNG, NA, ICR-170, as well as UV were used to induce mutations in the wild-type haploid strain X2180-1B (α) of Saccharomyces. A total of 2053 (EMS, 427; MNNG, 444; NA, 469; ICR-170, 456; UV, 257) lysine-requiring mutant clones were isolated from many independent treatments and by nystatin enrichment technique. Mutants were classified into various functional groups on the basis of complementation analysis with 14 tester strains (lys 1 to lys 15 except lys 3). Of the clones analyzed, the number of isolates unable to complement with a given tester strain ranged from 2 for lys 5 to 918 for lys 4. Three of the mutually complementing lysine loci (lys 1, lys 2, and lys 4) accounted together for over 85% of the mutant clones whereas lys 6, lys 7, lys 8, and lys 14 had less than 10 noncomplementing isolates each. Mutants for lys 4 were most frequent with all of the mutagens tested except with NA in which case the mutants for lys 2 were most frequent. A total of 56 isolates failed to complement with lys 10, lys 11, and lys 12. Similarly, 47 isolates failed to complement with lys 9 and lys 13 simultaneously. Only 44 isolates complemented with all of the tester strains used.

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Notes

  1. EMS: Ethyl methanesulfonate; MNNG: N-methyl-N\t'-nitro-N\t'-nitrosoguanidine; NA: Nitrous acid; ICR-170: 2-methoxy-6-chloro-9-[3-(ethyl-2-chloroethyl) aminopropylamino] acridine 2 HCl; UV: Ultra violet radiation.

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  1. Department of Microbiology, Miami University, 45056, Oxford, Ohio, USA

    G. D. Biswas & J. K. Bhattacharjee

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  1. G. D. Biswas
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  2. J. K. Bhattacharjee
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Biswas, G.D., Bhattacharjee, J.K. Induction and complementation of lysine auxotrophs in Saccharomyces . Antonie van Leeuwenhoek 40, 221–231 (1974). https://doi.org/10.1007/BF00394380

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