Summary
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1.
The spermatocytes and early spermatids of Vaginula possess a single Golgi element, consisting of an osmiophil externum and an osmiophobe internum. It is somewhat spherical, and is clearly observable in untreated living cells. In late spermatids the Golgi bodies break up into granules indistinguishable from mitochondria.
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2.
The acrosome is a small structure and arises without the intervention of the Golgi bodies.
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3.
The mitochondria are small osmiophil granules and rodlets. They remain scattered haphazard and do not give rise to Nebenkern or mitochondrial sheath of the middle piece.
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4.
The Golgi body does not consist of vacuome and ‘chondriome actif’. It stains neither with janus green B, nor with neutral red, but stains deeply with nile blue sulphate and methylene blue.
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5.
The mitochondria stain with janus green B, but not with nile blue sulphate.
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6.
Neutral-red bodies appear in the ground cytoplasm of the spermatocytes and spermatids. These are probably segregation droplets.
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Srivastava, M.D.L. Cytoplasmic organelles in the male germ-cells of Vaginula maculata (pulmonata, mollusca). Z.Zellforsch 40, 313–322 (1954). https://doi.org/10.1007/BF00394136
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DOI: https://doi.org/10.1007/BF00394136