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Folinic acid effect on 5-fluorouracil kinetics in vivo

  • Original Papers
  • Experimental Oncology
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Summary

Synergy of folinic acid and FU has been shown in several experimental systems. In the present study we examined the kinetics of concurrent administration of folinic acid on the kinetics of FU in an in vivo model. Folinic acid significantly increased the amount of FU bound to the TS complex. In the sequential combination of MTX and FU folinic acid had no additional effect. The amount of FU incorporated into RNA was not affected in any of the treatment groups. The results indicate that folinic acid may improve FU cytotoxicity by increased FU binding to TS but has no additional effect on the synergistic sequential MTX-FU combination

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Abbreviations

MTX:

methotrexate

FU:

5-fluorouracil

TS:

thymidylate synthetase

FdUMP (and UTP):

5-fluoro-2′-deoxyuridine, 5′-monophosphate (-triphosphate)

PRPP:

5-phosphoribosyl 1-pyrophosphate

CH2FAH4 :

N5,10-methylenetetrahydrofolate

DHFR:

dihydrofolate reductase

PCA:

perchloric acid

LCV:

leucovorin (folinic acid)

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Authors and Affiliations

  1. Medizinische Klinik des Klinikums Charlottenburg der Freien Universität Berlin, Berlin, Germany

    R. Herrmann, J. Reuter, R. Port & H. Osswald

  2. Deutsches Krebsforschungszentrum Heidelberg, Federal Republic of Germany

    R. Herrmann, J. Reuter, R. Port & H. Osswald

Authors
  1. R. Herrmann
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  2. J. Reuter
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  3. R. Port
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  4. H. Osswald
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Additional information

Dedicated to Professor Werner Kunz on the occasion of his 65th birthday

Supported in part by a grant from Tumorzentrum Heidelberg-Mannheim, FRG

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Herrmann, R., Reuter, J., Port, R. et al. Folinic acid effect on 5-fluorouracil kinetics in vivo. J Cancer Res Clin Oncol 114, 87–90 (1988). https://doi.org/10.1007/BF00390490

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  • DOI: https://doi.org/10.1007/BF00390490

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