Summary
Only 11% of the total peroxidase activity in a root tip homogenate of Zea mays (L.) was sedimentable. The majority of sedimentable activity was bound to membrane fractions with which acid hydrolases were also associated.
Peroxidase activity was released from a cell wall fraction exhaustively washed using homogenization and detergent. The specific activities of the washings were 3–10 fold higher than in the sedimentable and soluble fractions. A quarter of the wall associated activity was only released after treatment of the washed wall preparation with cellulase.
Histochemistry shows peroxidase associated with cell walls. The only activity within the cell was observed associated with the inner surface of the provacuole membranes. The provacuoles were observed fusing in maturing cells. No peroxidase was seen in the Golgi.
One peroxidase isozyme was apparently specifically associated with the sedimentable membrane fractions and may represent the activity observed in the provacuole membranes. Three fast migrating isozymes were restricted to the soluble fractions while at least three slow migrating isozymes were wall associated. One of the latter was specifically released from the wall by calcium nitrate. The relationship between the isozymes is discussed.
A scheme for the origin of vacuoles is proposed.
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References
Berjak, P.: Lysosomal compartimentation: ultrastructural aspects of the origin, development and function of vacuoles in root cells of Lepidium sativum. Ann. Bot. 36, 73–81 (1972)
Brown, S. A.: Chemistry of lignification. Science 134, 305–313 (1961)
Corbett, J. R., Price, C. A.: Localization of acid phosphatase in plant cells. Plant Physiol. 42, 827–830 (1967)
Czaninski, Y., Catesson, A.: Localisation ultrastructurale d'activités peroxydasiques dans les tissus conducteurs végétaux au cours du cycle annuel. J. Microscopie 8, 875–888 (1969)
Dauwalder, M., Whaley, W. C., Kephart, J. E.: Phosphatases and differentiation of the Golgi apparatus. J. Cell Sci. 4, 455–498 (1969)
Davis, B. J.: Dise Electrophoresis. Method and application to human serum proteins. Ann. N. Y. Acad. Sci. 121, 404–427 (1964)
De Duve, C.: The lysosome in retrospect. In: Lysosomes in virology and pathology (eds. J. T. Dingle and H. B. Fell), vol. 1., p. 3–40. Amsterdam-London: North-Holland Publishing Co. 1969
De Duve, C., Wattiaux, R.: Functions of lysosomes. Ann. Rev. Physiol. 28, 435–492 (1966)
Hall, J. L., Sexton, R.: Cytochemical localization of peroxidase activity in root cells. Planta (Berl.) 108, 103–120 (1972)
Lipetz, J., Garro, A. J.: Ionic effects on lignification and peroxidase in tissue cultures. J. Cell Biol. 25, 109–116 (1965)
Lowry, O. H., Rosenbrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin reagent. J. biol. Chem. 193, 265–275 (1951)
Lück, H.: Peroxidase. In: Methods of enzymatic analysis (ed. H. U. Bergmeyer), p. 895–897. New York: Academic Press 1963
Matile, Ph.: Lysosomes of root tip cells in corn seedlings. Planta (Berl.) 79, 181–196 (1968)
Matile, Ph., Moor, H.: Vacuolation: Origin and development of the lysosomal apparatus in root tip cells. Planta (Berl.) 80, 159–175 (1968)
Michell, R. H., Karnovsky, M. J., Karnovsky, M. L.: The distributions of some granule-associated enzymes in guinea-pig polymorphonuclear leucocytes. Biochem J. 116, 207–216 (1970)
Parish, R. W.: Studies on senescing tobacco leaf disks with special reference to peroxidase. I. The effects of inhibition of nucleic acid and protein synthesis. Planta (Berl.) 82, 1–13 (1968)
Parish, R. W.: Studies on wheat peroxidases. Aust. J. biol. Sci. 22, 261–266 (1969a)
Parish, R. W.: Effects of indoleacetic acid on lignification in wheat internodes and in vitro. Aust. J. biol. Sci. 22, 261–266 (1969b)
Parish, R. W.: Studies on the effect of calcium and boron on peroxidases of plant cell walls. Z. Pflanzenphysiol. 60, 211–216 (1969c)
Parish, R. W.: The isolation of peroxisomes, mitochondria and chloroplasts from leaves of spinach beet (Beta vulgaris L. ssp. vulgaris). Europ. J. Biochem. 22, 423–429 (1971)
Parish, R. W.: The intracellular location of phenol oxidases, peroxidase and phosphatases in the leaves of spinach beet (Beta vulgaris L. ssp. vulgaris). Europ. J. Biochem. 31, 446–455 (1972).
Parish, R. W., Miller, F. L.: The uptake and effects of calcium and phosphate on maturity, lignification and peroxidase activity of wheat internodes. Aust. J. biol. Sci. 22, 77–85 (1969)
Plesnicar, M., Bonner, W. D., Storey, B. T.: Peroxidase associated with higher plant mitochondria. Plant Physiol. 42, 366–370 (1967)
Poux, N.: Nouvelles observations sur la nature et l'origine de la membrane vacuolaire des cellules végétales. J. Microscopie 1, 55–66 (1962)
Poux, N.: Localisation d'activitiés enzymatiques dans les cellules du méristème radiculaire de Cucumis sativus L.. J. Microscopie 8, 855–866 (1969)
Reaveley, D. A., Rogers, H. J.: Some enzymic activities and chemical properties of the mesosomes and cytoplasmic membranes of Bacillus licheniformis 634 b. Biochem. J. 113, 67–79 (1969)
Ridge, I., Osborne, D. J.: Hydroxyproline and peroxidases in cell walls of Pisum sativum: regulation by ethylene. J. exp. Bot. 21, 843–856 (1970)
Villiers, T. A.: Lysosomal activities of the vacuole in damaged and recovering plant cells. Nature (Lond.) New Biol. 233, 57–58 (1971)
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Parish, R.W. The lysosome-concept in plants. Planta 123, 1–13 (1975). https://doi.org/10.1007/BF00388056
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DOI: https://doi.org/10.1007/BF00388056