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The isolation of barley-aleurone protoplasts

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Summary

A procedure is described for the isolation of large numbers of viable aleurone protoplasts. After treatment with “Onozuka” cellulase protoplasts were obtained which were surrounded by a thin, “Onozuka”-resistant wall. These cells were termed “spheroplasts”. Treatment of spheroplasts with “Glusulase” digested away the residual wall, yielding naked protoplasts. Measurements of respiration using a Clarke-type oxygen electrode indicated that aleurone cells isolated by this procedure were viable.

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References

  • Chrispeels, M. J., Varner, J. E.: Gibberellic acid-enhanced synthesis and release of α-amylase and ribonuclease by isolated barley aleurone layers. Plant Physiol. 42, 398–406 (1967).

    Google Scholar 

  • Filner, P., Varner, J. E.: A simple and unequivocal test for de novo synthesis of enzymes: Density labelling of barley α-amylase with H2O18. Proc. nat. Acad. Sci. (Wash.) 58, 1520–1526 (1967).

    Google Scholar 

  • Gamborg, O. L., Miller, R. A., Ojima, K.: Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50, 151–158 (1968).

    PubMed  Google Scholar 

  • Jacobsen, J. V., Varner, J. E.: Gibberellic acid-induced synthesis of protease by isolated aleurone layers of barley. Plant Physiol. 42, 1596–1600 (1967).

    Google Scholar 

  • Jensen, W. A.: A morphological and biochemical analysis of the early phases of cellular growth in the root tip of Vicia faba. Exp. Cell Res. 8, 506–522 (1955).

    PubMed  Google Scholar 

  • Jones, R. L.: The fine structure of barley aleurone cells. Planta (Berl.) 85, 359–375 (1969).

    Google Scholar 

  • Taiz, L., Jones, R. L.: Gibberellic acid, β-1,3-glucanase and the cell walls of barley aleurone layers. Planta (Berl.) 92, 73–84 (1970).

    Google Scholar 

  • Yomo, H.: Studies on the α-amylase activating substance. IV. On the amylase activating action of gibberellin. [In Jap.] Hakko Kyokaishi 18, 600–602 (1960).

    Google Scholar 

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Authors and Affiliations

  1. Department of Botany, University of California, Berkeley

    Lincoln Taiz & Russell L. Jones

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  1. Lincoln Taiz
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  2. Russell L. Jones
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Additional information

Work supported by National Science Foundation grant GB-27468.

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Taiz, L., Jones, R.L. The isolation of barley-aleurone protoplasts. Planta 101, 95–100 (1971). https://doi.org/10.1007/BF00387620

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  • DOI: https://doi.org/10.1007/BF00387620

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