Summary
The possible mechanism which leads to inactivation phenomena at high intensities of blue light during the color-sensitive phase in the time course of the Hill reaction was investigated in more detail. The following results were obtained: a) The inhibition of the Hill reaction is also observed during but not prior to the color-sensitive state if the supernatant of a blue-illuminated chloroplast suspension is used as a medium for a fresh assay in red light. b) Addition of linolenic acid, which leads to a stimulation of electron transport in intact chloroplasts, intensifies the inhibition at the blue-light-sensitive stage. c) The content of malondialdehyde, an indicator for free-radical-type breakdown of unsaturated fatty acids, increases selectively after blue-light exposure during the color-sensitive phase of the Hill reaction. This result was obtained with both the thiobarbituric-acid test and fluorescence measurements of Schiff-base type conjugates. d) The gas chromatograms of total chloroplast lipids show a different spectrum for plastids exposed to blue light when compared to a non-illuminated control sample. The changes imply alterations in the composition of long-chain fatty acids. These results warrant the conclusion that the blue-light inhibition is closely linked to fatty-acid breakdown which is sensitized by light absorbed by pigments detached from the photosynthetic centers during the process of chloroplast aging in vitro.
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References
Borst, P. J., Loos, A., Christ, E. J., Slater, E. D.: Uncoupling activity of long chain fatty acids. Biochim. biophys. Acta (Amst.) 62, 509–518 (1962).
Butler, R. D., Simon, E. D.: Ultrastructural aspects of senescence in plants. Adv. Geront. Res. 3, 73–129 (1971).
Chio, K. S., Reiss, U., Fletcher, B., Tappel, A. L.: Peroxidation of subcellular organelles: formation of lipofuscin-like fluorescent pigments. Science 166, 1535 (1969).
Dahle, L. K., Hill, E. G., Holman, R. T.: The thiobarbituric acid reaction and the autooxidation of polyunsaturated fatty acid methylesters. Arch. Biochem. 98, 253–261 (1962).
Goodman, D. S.: The interaction of human serum albumin with long chain fatty acids. J. Amer. chem. Soc. 80, 3892–3898 (1947).
Harnischfeger, G.: Changes in appearance, volume and activity during the early stages of disintegration in isolated chloroplasts. Planta (Berl.) 92, 164–177 (1970).
Harnischfeger, G., Gaffron, H.: Transient color sensitivity of the Hill reaction during the disintegration of chloroplasts. Planta (Berl.) 89, 383–388 (1969).
Harnischfeger, G., Gaffron, H.: Transient light effects in the Hill reaction of disintegrating chloroplasts in vitro. Planta (Berl.) 93, 89–105 (1970).
Heath, R. L., Packer, L.: Photoperoxidation in isolated chloroplasts. I. Kinetics and stochiometry of fatty acid peroxidation. Arch. Biochem. 125, 189–198 (1968).
Heath, R.L., Packer, L.: Photoperoxidation in isolated chloroplasts. II. Role of energy transfer. Arch. Biochem. 125, 850–857 (1968).
Packer, L., Deamer, D.W., Heath, R. L.: Regulation and deterioration of structure in membranes. Adv. Geront. Res. 2, 77–120 (1967).
Pohl, P., Glasl, H., Wagner, H.: Zur Analytik pflanzlicher Glyko- und Phospholipide und ihrer Fettsäuren. I. Eine neue dünnschichtchromatographische Methode zur Trennung pflanzlicher Lipoide und quantitativen Bestimmung ihrer Fettsäurezusammensetzung. J. Chromatography 49, 488–492 (1972).
Satoh, K.: Mechanism of photoinactivation in photosynthetic systems. I. The dark reaction in photoinactivation. Plant Cell Physiol. 11, 15–28 (1970a).
Satoh, K.: Mechanisms of photoinactivation in photosynthetic systems. II. The occurrence and properties of two different types of photoinactivation. Plant Cell Physiol. 11, 29–38 (1970b).
Siegenthaler, P. A.: Chloroplast aging in vitro and relationships to fatty acids and polyphenol-oxidase activity. Experientia (Basel) 26, 1308–1310 (1970).
Tarladgis, B. G., Pearson, A. M., Dugan, L. R., Jr.: Chemistry of the 2-thiobarbituric acid test for determination of oxidative rancidity in foods. II. Formation of the TBA-malonaldehyde complex without acid heat treatment. J. Sci. Food Agric. 15, 602–607 (1964).
Wintermans, J. F. G. M., Helmsing, P. J., Polman, B. J. J., van Gisbergen, J., Colard, J.: Galactolipid transformations and photochemical activities of spinach chloroplasts. Biochim. biophys. Acta (Amst.) 189, 95–105 (1969).
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Supported in part by grant NGR10-004-018 of the National Aeronautics and Space Administration (NASA) to Dr. Hans Gaffron and in part by the Bundesministerium für Bildung and Wissenschaft of the Federal Republic of Germany.
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Harnischfeger, G. Photosensitized inhibitor formation in isolated, aging chloroplasts. Planta 104, 316–328 (1972). https://doi.org/10.1007/BF00386315
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DOI: https://doi.org/10.1007/BF00386315