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Protein kinase C activates the non-selective cation channel in the rabbit portal vein

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Abstract

Effects of protein kinase C (PKC) on a non-selective cation channel current (I ns) were investigated using smooth-muscle cells of the rabbit portal vein. Neither bath application of the PKC activator, phorbol 12,13-dibutyrate (PDBu; 1 μM), nor the internal application of guanosine 5′-[γ-thio]-triphosphate (GTP[γS]; 3 μM) elicited any current at the holding potential of −60 mV. However, when GTP[γS] (3 μM) was present in the pipette, PDBu elicited a sustained inward current, in a concentration-dependent manner, at the holding potential of −60 mV. On the other hand, an inactive phorbol ester, 4α-phorbol 12,13-didecanoate (300 nM and 1 μM) had no effect on the membrane current even when GTP[γS] (3 μM) was in the pipette. The current amplitude induced by PDBu in the presence of GTP[γS] in the pipette was markedly reduced following pretreatment with 10 μM staurosporine, a PKC inhibitor. Neither a reduction in the Cl− concentration in the pipette nor addition of niflumic acid to the bath inhibited the inward current, and the reversal potential estimated from the current/voltage relationship was about −5 mV (physiological salt solution containing 5 mM Ba2+/high CSCl), which revealed that the main component of the current is I ns. An internal application of pertussis toxin markedly reduced the amplitude of I ns induced by PDBu. These results indicate that PKC activates a sustained component of I ns in cooperation with an activated pertussis-toxin-sensitive G protein in the rabbit portal vein.

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References

  1. Barbacid M (1987) ras genes. Annu Rev Biochem 56:779–827

    Google Scholar 

  2. Benham CD (1989) ATP-activated channels gate calcium entry in single smooth muscle cells dissociated from rabbit ear artery. J Physiol (Lond) 419:689–701

    Google Scholar 

  3. Benham CD, Tsien RW (1987) Receptor-operated, Ca-permeable channels activated by ATP in arterial smooth muscle. Nature 328:275–278

    Google Scholar 

  4. Birnbaumer L, Abramowitz J, Brown AM (1990) Receptor-effector coupling by G-proteins. Biochem Biophys Acta 1031:163–224

    Google Scholar 

  5. Byrne NG, Large WA (1988) Mechanism of action of α-adrenoceptor activation in single cells freshly dissociated from the rabbit portal vein. Br J Pharmacol 94:475–482

    Google Scholar 

  6. Fish RD, Sperti G, Colucci WS, Clapham DE (1988) Phorbol ester increases the dihydropyridine-sensitive calcium conductance in a vascular smooth muscle line. Circ Res 62:1049–1054

    Google Scholar 

  7. Gilman AG (1987) G proteins: transducers of receptor-generated signals. Annu Rev Biochem 56:614–649

    Google Scholar 

  8. Gleason MM, Flaim F (1986) Phorbol ester contracts rabbit thoratic aorta by increasing intracellular calcium and by activating calcium influx. Biochem Biophys Res Commun 138:1362–1369

    Google Scholar 

  9. Hamill OP, Marty A, Neher E, Sakman B, Sigworth FJ (1981) Improved patch-clamp techniques for high resolution current recording from cells and cell-free membrane patches. Pflügers Arch 391:85–100

    Google Scholar 

  10. Inoue R, Isenberg G (1990) Intracellular calcium ions modulate acetylcholine-induced inward current in guinea-pig ileum. J Physiol (Lond) 424:73–92

    Google Scholar 

  11. Inoue R, Kitamura K, Kuriyama H (1987) Acetylcholine activates single sodium channels in smooth muscle cells. Pflügers Arch 410:69–74

    Google Scholar 

  12. Khalil RA, van Breemen C (1988) Sustained contraction of vascular smooth muscle: Calcium influx or C-kinase activation? J Pharmacol Exp Ther 244:537–542

    Google Scholar 

  13. Komori S, Bolton TB (1990) Role of G-proteins in muscarinic receptor inward and outward currents in rabbit jejunal smooth muscle. J Physiol (Lond) 427:395–419

    Google Scholar 

  14. Komori S, Bolton TB (1991) Calcium release induced by inositol 1,4,5-trisphosphate in single rabbit intestinal smooth muscle cells. J Physiol (Lond) 433:495–517

    Google Scholar 

  15. Loirand G, Pacaud P, Mironneau C, Mironneau J (1990) GTP-binding proteins mediate noradrenaline effects on calcium and chloride currents in rat portal vein myocytes. J Physiol (Lond) 428:517–529

    Google Scholar 

  16. Markel LA, Rivera LM, Colussi DJ, Perrone MH (1991) Protein kinase C and vascular smooth muscle contractility: effects of inhibitors and down-regulation. J Pharmacol Exp Ther 257:134–140

    Google Scholar 

  17. Momose K, Gomi Y (1980) Studies on isolated smooth muscle cells. IV. Dispersion procedures for acetylcholine-sensitive smooth muscle cells of guinea-pig (abstract in English). Jpn J Smooth Muscle Res 6:29–36

    Google Scholar 

  18. Nishizuka Y (1988) The molecular heterogeneity of protein kinase C and its implications for cellular regulation. Nature 334:661–665

    Google Scholar 

  19. Ohya Y, Terada K, Yamaguchi K, Inoue R, Okabe K, Kitamura K, Hirata M, Kuriyama H (1988) Effects of inositol phosphates on the membrane activity of smooth muscle cells of the rabbit portal vein. Pflügers Arch 412:382–389

    Google Scholar 

  20. Oike M, Kitamura K, Kuriyama H (1992) Histamine H3-receptor activation augments voltage-dependent Ca2+ current via GTP hydrolysis in rabbit saphenous artery. J Physiol (Lond) 448:133–152

    Google Scholar 

  21. Pacaud P, Loirand G, Lavine JL, Mironneau C, Mironneau J (1989) Calcium-activated chloride current in rat vascular smooth muscle cells in short-term primary culture. Pflügers Arch 413:629–636

    Google Scholar 

  22. Saigusa A, Kokubun S (1988) Protein kinase C may regulate resting anion conductance in vascular smooth muscle cells. Biochem Biophys Res Commun 155:882–889

    Google Scholar 

  23. Shearman MS, Sekiguchi K, Nishizuka Y (1989) Modulation of ion channel: a key function of the protein kinase C enzyme family. Pharmacol Rev 41:211–237

    Google Scholar 

  24. Singer HA (1990) Phorbol ester-induced stress and myosin light chain phosphorylation in swine carotid medial smooth muscle. J Pharmacol Exp Ther 252:1068–1074

    Google Scholar 

  25. Suematsu E, Hirata M, Hashimoto T, Kuriyama H (1984) Inositol 1,4,5-trisphosphate releases Ca2+ from intracellular store sites in skinned single cells of porcine coronary artery. Biochem Biophys Res Commun 120:481–485

    Google Scholar 

  26. Tamaoki T, Nomoto H, Takahashi I, Kato Y, Morimoto M, Tomita F (1986) Staurosporine, a potent inhibitor of phospho-lipid/Ca2+ dependent protein kinase. Biochem Biophys Res Commun 135:397–402

    Google Scholar 

  27. Terada K, Kitamura K, Kuriyama H (1987) Blocking actions of Ca2+ antagonists on the Ca2+ channels in the smooth muscle cell membrane of rabbit small intestine. Pflügers Arch 408:552–557

    Google Scholar 

  28. Xiong ZL, Kitamura K, Kuriyama H (1991) ATP activates cationic currents and modulates the calcium current through GTP-binding protein in rabbit portal vein. J Physiol (Lond) 440:143–165

    Google Scholar 

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Authors and Affiliations

  1. Department of Pharmacology, Faculty of Medicine, Kyushu University, 812, Fukuoka, Japan

    Masahiro Oike, Kenji Kitamura & Hirosi Kuriyama

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  1. Masahiro Oike
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  2. Kenji Kitamura
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  3. Hirosi Kuriyama
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Oike, M., Kitamura, K. & Kuriyama, H. Protein kinase C activates the non-selective cation channel in the rabbit portal vein. Pflugers Arch. 424, 159–164 (1993). https://doi.org/10.1007/BF00374607

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  • DOI: https://doi.org/10.1007/BF00374607

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