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Kinetic mode switch of rat brain IIA Na channels in Xenopus oocytes excised macropatches

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Abstract

Na currents recorded from inside-out macropatches excised from Xenopus oocytes expressing the α subunit of the rat brain Na channel IIA show at least two distinguishable components in their inactivation time course, with time constants differing about tenfold (τ h1 = approx. 150 μs and τ h2 = approx. 2 ms). In excised patches, the inactivation properties of Na currents changed with time, favoring the faster inactivation kinetics. Analysis of the fast and slow current kinetics shows that only the relative magnitudes of τ h1 and τ h2 components are altered without significant changes in the time constants of activation or inactivation. In addition, voltage dependence of both activation and steady-state inactivation of Na currents are shifted to more negative potentials in patches with predominantly fast inactivation, although reversal potentials and valences remained unaltered. We conclude that the two inactivation modes discerned in this study are conferred by two states of Na channel the interconversion of which are regulated by an as yet unknown mechanism that seems to involve cytosolic factors.

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  1. Andrea Fleig

    Present address: Department of Membrane Biophysics, Max-Planck-Institute for Biophysical Chemistry, Am Fassberg, D-37077, Göttingen, Germany

Authors and Affiliations

  1. Department of Physiology, John A. Burns School of Medicine, University of Hawaii, 96822, Honolulu, HI, USA

    Andrea Fleig & Martin D. Rayner

  2. Békésy Laboratory of Neurobiology, University of Hawaii, 96822, Honolulu, HI, USA

    Peter C. Ruben

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  2. Peter C. Ruben
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Fleig, A., Ruben, P.C. & Rayner, M.D. Kinetic mode switch of rat brain IIA Na channels in Xenopus oocytes excised macropatches. Pflügers Arch. 427, 399–405 (1994). https://doi.org/10.1007/BF00374253

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  • DOI: https://doi.org/10.1007/BF00374253

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