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Modulation of the FMRFamide-gated Na+ channel by external Ca2+

  • Ion Channels, Receptors and Transporters
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Abstract

FMRFamide-gated Na+ channel (FaNaC) is a member of the DEG/ENaC family. Amino acid sequence of the second transmembrane region (TM2) of FaNaC is quite similar to that of the acid-sensing ion channels (ASIC) of the same family. In the upper part of TM2, there are two aspartate residues (D552 and D556 in Aplysia FaNaC, AkFaNaC) which construct two negative rings in the external vestibule. In the present study, we examined the function of D552/D556 mutants of AkFaNaC in Xenopus oocytes with special interest in Ca2+ sensitivity of FaNaC. The FMRFamide-evoked current through AkFaNaC was depressed by submillimolar Ca2+ such that the current in Ca2+-free condition was 2–3-fold larger than that in the control solution which contained 1.8 mM CaCl 2. Both D552 and D556 were found to be indispensable for the sensitivity of FaNaC to submillimolar Ca2+. Unexpectedly, however, both acidic residues were not essential for the inhibition by millimolar Ca2+ concentrations. The Ca2+-sensitive gating of FaNaC was recapitulated by an allosteric model in which Ca2+-bound channels are more difficult to open. The desensitization of FaNaC was also inhibited by Ca2+, which was abolished in some D552/D556 mutants. Structural models of FaNaC made by homology modeling showed that the distance between oxygen atoms of D552 and D556 on the adjacent subunits is close enough to coordinate Ca2+ in the nonconducting desensitized channel but not in the open channel. The results suggest that Ca2+ coordination between oxygen atoms of D552 and D556 disturbs the opening transition as well as the desensitization of FaNaC.

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Acknowledgments

This work was supported by JSPS KAKENHI Grant Number JP15K07149.

Author contributions

YF conceived research; YF designed experiments; AF and YK performed experiments; AF, YK, and YF analyzed data; YF performed the simulation and the homology modeling; YF wrote a manuscript; AF, YK, and YF approved the final version of the manuscript.

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Author notes

  1. Yu Kodani

    Present address: Department of Physiology, Fujita Health University School of Medicine, Toyoake, Aichi, 470-1192, Japan

Authors and Affiliations

  1. Laboratory of Neurobiology, Graduate School of Integrated Arts and Sciences, Hiroshima University, Kagamiyama 1-7-1, Higashi-Hiroshima, 739-8521, Japan

    Akihiko Fujimoto, Yu Kodani & Yasuo Furukawa

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  1. Akihiko Fujimoto
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  2. Yu Kodani
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  3. Yasuo Furukawa
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Correspondence to Yasuo Furukawa.

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This article is part of the Topical Collection on Ion channels, receptors and transporters

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Fujimoto, A., Kodani, Y. & Furukawa, Y. Modulation of the FMRFamide-gated Na+ channel by external Ca2+ . Pflugers Arch - Eur J Physiol 469, 1335–1347 (2017). https://doi.org/10.1007/s00424-017-2021-z

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