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Principal cells of cortical collecting ducts of the rat are not a route of transepithelial Cl− transport

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Abstract

The rat cortical collecting duct (CCD) exhibits high rates of NaCl reabsorption when stimulated by mineralocorticoid and antidiuretic hormone (ADH). The present study was undertaken to determine if there is significant transcellular Cl− movement across the principal cells of the rat CCD. CCDs were dissected from kidneys of rats that had been injected with deoxycorticosterone (5 mg, i.m.) 2–9 days prior to the experiment. The ducts were perfused in vitro with identical perfusing and bathing solutions, except that 200 pmol.l−1 ADH was added to the bathing solutions. The basolateral membrane voltage (PDbl) of principal cells was −77±1 mV and the luminal membrane voltage (PD1) was −68±1 mV (mean ± SEM, n=124). Separate impalements with single-barrelled Cl−-selective microelectrodes gave an apparent intracellular Cl− activity of principal cells of 17±2 mmol.l−1. Transepithelial PD and PDbl were unaffected by luminal furosemide, hydrochlorothiazide (HCT), 4-acetamido-4-isothiocyanostilbene2,2-disulphonic acid, (SITS), or the Cl− channel blocker 5-nitro-2-(3-phenylpropylamino)-benzoic acid (NPPB); bath addition of SITS or the Cl− channel blocker diphenylamino-2-carboxylic acid; or replacement of bath HCO −3 by Cl−. The intracellular Cl− activity (a Clcell ) also remained unchanged with the addition of HCT, SITS or the Cl− channel blockers to either the perfusing or bathing solutions, or with replacement of the bathing solution HCO −3 . With Cl− replacement in both solutions, a Clcell decreased to 9 mmol.l−1, but not until after 4–6 min, indicating a very low rate of Cl− transport in these cells, even under conditions of maximal stimulation of NaCl reabsorption by mineralocorticoid plus ADH. The remaining a Clcell could be attributed to interference with the Cl− selective electrodes by other cytosolic anions. We conclude that a Clcell of principal cells in the rat CCD is not far above passive equilibrium, and that these cells do not contribute significantly to transepithelial Cl− reabsorption, which must occur by alternative routes such as the paracellular pathway, and/or through intercalated cells.

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References

  1. Atkins JL, Burg MB (1985) Bicarbonate transport by isolated perfused rat collecting ducts. Am J Physiol 249: F485-F489

    Google Scholar 

  2. Greger R (1981) Cation selectivity of the isolated perfused cortical thick ascending limb of Henle's loop of rabbit kidney. Pflügers Arch 390: 30–37

    Google Scholar 

  3. Greger R, Hampel W (1981) A modified system for in vitro perfusion of isolated renal tubules. Pflügers Arch 389: 175–176

    Google Scholar 

  4. Greger R, Schlatter E (1983) Properties of the lumen membrane of the cortical thick ascending limb of Henle's loop of rabbit kidney. Pflügers Arch 396: 315–324

    Google Scholar 

  5. Greger R, Schlatter E (1984) Mechanism of NaCl secretion in the rectal gland of spiny dogfish (Squalus acanthisa). I. Experiments in isolated in vitro perfused rectal gland tubules. Pflügers Arch 402: 63–75

    Google Scholar 

  6. Greger R, Schlatter E (1984) Mechanism of NaCl secretion in rectal gland tubules of spiny dogfish (Squalus acanthias). II. Effects of inhibitors. Pflügers Arch 402: 364–375

    Google Scholar 

  7. Greger R, Oberleithner H, Schlatter E, Cassola AC, Weidtke C (1983) Chloride activity in cells of isolated perfused cortical thick ascending limbs of rabbit kidney. Pflügers Arch 399: 29–34

    Google Scholar 

  8. Greger R, Schlatter E, Lang F (1983) Evidence for electroneutral sodium chloride cotransport in the cortical thick ascending limb of Henle's loop of rabbit kidney. Pflügers Arch 396: 308–314

    Google Scholar 

  9. Greger R, Schlatter E, Wang F, Forrest JNJr (1984) Mechanism of NaCl secretion in rectal gland tubules of spiny dogfish (Squalus acanthias). III. Effects of stimulation of secretion by cyclic AMP. Pflügers Arch 402: 376–384

    Google Scholar 

  10. Madsen KM, Verlander JW, Tisher CC (1988) Relationship between structure and function in distal tubule and collecting duct. J Electron Microsc Techn 9: 187–208

    Google Scholar 

  11. O'Neil RG, Boulpaep E (1982) Ionic conductive properties and electrophysiology of the rabbit cortical collecting tubule. Am J Physiol 243: F81-F95

    Google Scholar 

  12. O'Neil RG, Hayhurst RA (1985) tFunctional differentiation of cell types of cortical collecting duct. Am J Physiol 248: F449-F453

    Google Scholar 

  13. O'Neil RG, Sansom SC (1984) Electrophysiological properties of cellular and paracellular conductive pathways of the rabbit cortical collecting duct. J Membr Biol 82: 281–295

    Google Scholar 

  14. Reif MC, Troutman SL, Schafer JA (1984) Sustained response to vasopressin in isolated rat cortical collecting tubule. Kidney Int 26: 725–732

    Google Scholar 

  15. Reif MC, Troutman SL, Schafer JA (1986) Sodium transport by rat cortical collecting ubule. Effects of vasopressin and desoxycorticosterone. J Clin Invest 77: 1291–1298

    Google Scholar 

  16. Sansom SC, Weinman EJ, O'Neil RG (1984) Microelectrode assessment of chloride-conductive properties of cortical collecting duct. Am J Physiol 247: F291-F302

    Google Scholar 

  17. Sauer M, Dörge A, Thurau K, Beck F-X (1989) Effect of ouabain on electrolyte concentrations in principal and intercalated cells of the isolated perfused cortical collecting duct. Pflügers Arch 413: 651–655

    Google Scholar 

  18. Schafer JA, Troutman SL (1986) Effect of ADH on rubidium transport in isolated perfused rat cortical collecting tubules. Am J Physiol 250: F1063-F1072

    Google Scholar 

  19. Schafer JA, Troutman SL (1987) Potassium transport in cortical collecting tubules from mineralocorticoid-treated rat. Am J Physiol 253: F76-F88

    Google Scholar 

  20. Schafer JA, Troutman SL, Schlatter E (1990) Vasopressin and mineralocorticoid increase apical membrane driving force for K+ secretion in rat CCD. Am J Physiol 258: F199-F210

    Google Scholar 

  21. Schlatter E (1989) Antidiuretic hormone regulation of electrolyte transport in the distal nephron. Renal Physiol Biochem 12: 65–84

    Google Scholar 

  22. Schlatter E, Greger R (1985) cAMP increases the basolateral Cl−-conductance in the isolated perfused medullary thick ascending limb of Henle's loop of the mouse. Pflügers Arch 405: 367–376

    Google Scholar 

  23. Schlatter E, Schafer JA (1987) Electrophysiological studies in principal cells of rat cortical collecting tubules. ADH increases the apical membrane Na+-conductance. Pflügers Arch 409: 81–92

    Google Scholar 

  24. Schlatter E, Schafer JA (1988) Cortical collecting duct cation transport. In: Davison AM (ed) Nephrology I. Bailliere Tindall, London, pp 294–303

    Google Scholar 

  25. Schlatter E, Bleich M, Greger R (1990) Ion channels in the luminal membrane of isolated perfused rat cortical collecting ducts (CCD). Pflügers Arch 415: R14

    Google Scholar 

  26. Schuster VL (1985) Bradykinin and vasopressin actions on rabbit cortical collecting tubule: mechanism of their interaction and effects on Na transport. Am J Physiol 249: F645-F653

    Google Scholar 

  27. Schuster VL (1986) Cyclic adenosine monophosphate-stimulated anion transport in rabbit cortical collecting duct. Kinetics, stoichiometry, and conductive pathways. J Clin Invest 78: 1621–1630

    Google Scholar 

  28. Schuster VL, Stokes JB (1987) Chloride transport by the cortical and outer medullary collecting duct. Am J Physiol 253: F203-F212

    Google Scholar 

  29. Schuster VL, Bonsip SM, Jennings ML (1986) Two types of collecting duct mitochondria-rich (intercalated) cells: lectin and band 3 cytochemistry. Am J Physiol 251: C347-C355

    Google Scholar 

  30. Stanton B, Puglisi E, Gellai M (1987) Localization of α 2-adrenoreceptor-mediated increase in renal Na+, K+ and water excretion. Am J Physiol 252: F1016-F1021

    Google Scholar 

  31. Stoner LC, Burg MB, Orloff J (1974) Ion transport in cortical collecting tubule; effect of amiloride. Am J Physiol 227: 453–459

    Google Scholar 

  32. Strange K (1989) Ouabain-induced cell swelling in rabbit cortical collecting tubule: NaCl transport by principal cells. J Membr Biol 107: 249–261

    Google Scholar 

  33. Tago K, Schuster VL, Stokes JB (1986) Stimulation of chloride transport by HCO3-CO2 in rabbit cortical collecting tubule. Am J Physiol 251: F49-F56

    Google Scholar 

  34. Tago K, Schuster VL, Stokes JB (1986) Regulation of chloride self exchange by cAMP in cortical collecting tubule. Am J Physiol 251: F40-F48

    Google Scholar 

  35. Tomita K, Pisano JJ, Knepper MA (1985) Control of sodium and potassium transport in the cortical collecting duct of the rat. Effects of bradykinin, vasopressin and deoxycorticosterone. J Clin Invest 76:132–136

    Google Scholar 

  36. Tomita K, Pisano JJ, Burg MB, Knepper MA (1986) Effects of vasopressin and bradykinin on anion transport by the rat cortical collecting duct. Evidence for an electroneutral sodium chloride transport pathway. J Clin Invest 77: 136–141

    Google Scholar 

  37. Wagner S, Vogel R, Lietzke R, Koob R, Drenckhahn D (1987) Immunochemical characterization of a band 3-like anion exchanger in collecting duct of human kidney. Am J Physiol 253: F213-F221

    Google Scholar 

  38. Wangemann P, Wittner M, Di Stefano A, Lang HJ, Englert HC, Schlatter E, Greger R (1986) Chloride channel blockers in the thick ascending limb. Pflügers Arch 406: R59

    Google Scholar 

  39. Warden DH, Schuster VL, Stokes JB (1986) The paracellular pathway of rabbit cortical collecting tubule (CCT): a high resistance, non-selective barrier. Fed Proc 45: 517

    Google Scholar 

  40. Warden DH, Schuster VL, Stokes JB (1988) Characteristics of the paracellular pathway of rabbit cortical collecting duct. Am J Physiol 255: F720-F727

    Google Scholar 

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Authors and Affiliations

  1. Physiologisches Institut, Albert-Ludwigs-Universität, W-7800, Freiburg, Federal Republic of Germany

    Eberhard Schlatter & Rainer Greger

  2. Department of Physiology and Biophysics, Nephrology Research and Training Center, University of Alabama at Birmingham, 35294, Birmingham, AL, USA

    James A. Schafer

Authors
  1. Eberhard Schlatter
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  2. Rainer Greger
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  3. James A. Schafer
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Schlatter, E., Greger, R. & Schafer, J.A. Principal cells of cortical collecting ducts of the rat are not a route of transepithelial Cl− transport. Pflugers Arch. 417, 317–323 (1990). https://doi.org/10.1007/BF00370998

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  • DOI: https://doi.org/10.1007/BF00370998

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