Abstract
Proteins of free and polysome-derived ribosomal subunits of Bacillus licheniformis were fractionated by means of two-dimensional polyacrylamide gel electrophoresis. The free and derived 30S subunits have twelve protein components in common. Five proteins are present on the free 30S subunits only, whereas four proteins are exclusively found on the derived 30S subunits. The free and derived 50S subunits have at least twenty-eight proteins in common. Four proteins are unique for the derived and one for the free 50S subunits.
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References
SchlessingerD. and ApirionD., Ann. Rev. Microbiol. 23, 387 (1969).
NomuraM., Bacteriol. Rev. 34, 228 (1970).
WittmannH. G. and StöfflerG., in L.Bosch (ed.), The Mechanism of Protein Synthesis and its Regulation, North-Holland Publishing Company, Amsterdam-London, 1972, chapter 10.
KurlandC. G., Science 169, 1171 (1970).
VoynowP. and KurlandC. G., Biochemistry 10, 517 (1971).
WeberH. J., Molec. Gen. Genetics 119, 233 (1972).
MoraG., DonnerD., ThammanaP., LutterL., and KurlandC. G., Molec. Gen. Genetics 112, 229 (1971).
VanDijk-SalkinojaM. S., StoofT. J., and PlantaR. J., Eur. J. Biochem. 12, 474 (1970).
FogelS., and SypherdP. S., J. Bacteriol. 96, 358 (1968).
GoldA. M., Methods Enzymol. 11, 706 (1967).
KaltschmidtE. and WittmannH. G., Anal. Biochem. 36, 401 (1970).
Van derZeistB. A. M. and BultH., Eur. J. Biochem. 28, 463 (1972).
VanDijk-SalkinojaM. S. and PlantaR. J., FEBS Letters 12, 9 (1970).
VanDuinJ., VanKnippenbergP. H., DiebenM., and KurlandC. G., Molec. Gen. Genetics 116, 181 (1972).
Parenti-RosinaR., EisenstadtA., and EisenstadtJ. M., Nature 221, 363 (1969).
RevelM., HerzbergM., and GreenspanH., Cold Spring Harbor Symp. Quant. Biol. 34, 261 (1969)
BrawermanG., Cold Spring Harbor Symp. Quant. Biol. 34, 307 (1969).
DubnoffJ. S., LockwoodA. H., and MaitraU., Arch. Biochem. Biophys. 149, 528 (1972).
CravenG. R., VoynowP., HardyS. J. S., and KurlandC. G., Biochemistry 8, 2906 (1969).
DzionaraM., KaltschmidtE., and WittmannH. G., Proc. Nat. Ac. Sci. U.S. 67, 1909 (1970).
Brouwer, J. and Planta, R. J., 9th Int. Congress of Biochemistry, Abstract 3d19.
Bickle, T. A., Howard, G. A, and Traut, R. R., 9th Int. Congress of Biochemistry, Abstract 3g10.
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Brouwer, J., Planta, R.J. Structural heterogeneity of 30S and 50S ribosomal subunits in Bacillus licheniformis . Molecular Biology Reports 1, 135–142 (1973). https://doi.org/10.1007/BF00357153
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DOI: https://doi.org/10.1007/BF00357153