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Ultrastructure et histochimie de la cuticule pharyngienne chez Eulalia viridis Müller, (Annélide Polychète Errante, Phyllodocidae)

Ultrastructure and histochemistry of the pharyngeal cuticle of Eulalia viridis (Müller) (Polychaeta Errantia, Phyllodocidae) and its relationships with the subjacent epithelium in the digestive cycle

Étude de ses rapports avec l'épithélium sous-jacent dans le cycle digestif

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Summary

The pharyngeal epithelium of Eulalia viridis (Müller) is covered with a thick cuticle and is built up by two types of glandular cells: 1) serous cells secreting inactive proteolytique enzymes which are perhaps activated, on the outside of the cuticle by the acid secretion of the 2) fluteshaped cells. The digestive process was followed by controlled feeding experiments and investigated with the light- and electron-microscope. The pharyngeal cuticle consists of two main zones. The cuticle proper is formed by collagenous fibers — orientated in two orthogonal directions — which are perforated by epithelial microvilli. The epicuticle is formed by the tips of the microvilli covered by an acid mucous filamentous coat which can be compared to a glycocalyx.

Resumé

L'épithélium pharyngien d'Eulalia viridis (Müller), recouvert d'une épaisse cuticule, comporte deux catégories de cellules glandulaires: les cellules séreuses sécrètent des proferments protéolytiques qui sont peut-etre activés à l'extérieur de la cuticule par la sécrétion acide des cellules en forme de flûtes. Le processus digestif a été suivi par des expériences d'alimentation contrôlée, vérifiées en microscopie optique et électronique. La cuticule pharyngienne est constituée de deux zones principales: la cuticule proprement dite, formée de fibres de collagène dirigées dans deux directions perpendiculaires l'une à l'autre et traversée par des microvillosités épithéliales; l'épicuticule constituée par les extrémités renflées des microvillosités épithéliales recouvertes d'un revêtement filamenteux à mucopolysaccharides acides non sulfatés comparable à un glycocalyx.

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Bibliographie

  • Bennett, S. H.: Morphological aspects of extracellular polysaccharides. J. Histochem. Cytochem. 11, 14–24 (1963).

    Google Scholar 

  • Boilly, B.: Contribution à l'étude ultrastructurale de la cuticule épidermique et pharyngienne chez une Annélide Polychète (Syllis amica Quatrefages). J. Micr. 6, 469–484 (1967).

    Google Scholar 

  • —: Nature des formations tabulaires épicuticulaires de Syllis amica Q. (Annélide Polychète). J. Micr. 7, 931–933 (1968).

    Google Scholar 

  • Brandt, Ph. D.: A consideration of the extraneous coats of the plasma membrane. Circulation 26, 1075–1091 (1962).

    Google Scholar 

  • —, and G. D. Pappas: An electron microscopic study of pinocytosis in Amoeba, I The surface attachment phase. J. biophys. biochem. Cytol. 8, 675–687 (1960).

    Google Scholar 

  • Brökelmann, J., u. A. Fischer: Über die Cuticula von Platynereis dumerilii (Polychaeta). Z. Zellforsch. 70, 131–135 (1966).

    Google Scholar 

  • Chévremont, M.: Notions de Cytologie et Histologie. Liège: Desoer (1960).

    Google Scholar 

  • Coggeshall, R. E.: A fine structural analysis of the epidermis of the earthworm Lumbricus terrestris L. J. Cell Biol. 28, 95–108 (1966).

    Google Scholar 

  • Curgy, J.-J.: Influence du mode de fixation sur la possibilité d'observer des structures myéliniques dans les hépatocytes d'embryons de Poulet. J. Micr. 7, 63–80 (1968).

    Google Scholar 

  • Fischer, A.: Über den Bau und die Hell-Dunkel Adaptation der Augen des Polychaeten Platynereis dumerilii. Z. Zellforsch. 61, 338–353 (1963).

    Google Scholar 

  • Hale, C. W.: Histochemical demonstration of acid polysaccharides in animal tissues. Nature (Lond.) 157, 802 (1946).

    Google Scholar 

  • Hess, R. T., and D. B. Menzell: The fine structure of the epicuticular particles of Enchytraeus fragmentosus. J. Ultrastruct. Res. 19, 487–497 (1967).

    Google Scholar 

  • Hladik, C. M.: Une structure nouvelle à la surface de lépithélium intestinal: la membrane du glycocalyx. C. R. Acad. Sci. (Paris) 263, 972–975 (1966).

    Google Scholar 

  • Ito, S.: The enteric surface coat on cat intestinal microvilli. J. Cell Biol. 27, 475–491 (1965).

    Google Scholar 

  • Jeuniaux, Ch.: Chitine et chitinolyse. Paris: Masson & Cie. 1963.

    Google Scholar 

  • Krall, J. F.: The cuticle and epidermal cells of Dero obtusa (Family Naïdidae). J. Ultrastruct. Res. 25, 84–93 (1968).

    Google Scholar 

  • Lillie, R. D.: Histopathologic technic and practical histochemistry. New York: Blakiston Co. 1954.

    Google Scholar 

  • Lison, L.: Histochimie et cytochimie animales. Principes et méthodes. Paris: Gauthiers-Villars 1960.

    Google Scholar 

  • Michel, C.: Cytologie et histochimie des glandes prépharyngiennes et du pharynx d'Eulalia viridis (Müller) (Phyllodocidae). Bull. Soc. zool. Fr. 90, 57–66 (1965).

    Google Scholar 

  • —: Comparaison du pharynx et des masses prépharyngiennes d'Eulalia viridis (Müller) et de Phyllodoce mucosa (Oersted) (Polychètes Errantes, Phyllodocidae). Arch. Zool. exp. gén. 107, 349–356 (1966).

    Google Scholar 

  • —: Enzymes digestives de la trompe d'Eulalia viridis (MÜller) (Phyllodocidae) et de Glycera convoluta (Keferstein) (Glyceridae) Annélides Polychètes Errantes. Ann. Histochim. 13, 123–134 (1968).

    Google Scholar 

  • - Enzymes digestives intestinales d'Eulalia viridis (Müller) (Phyllodocidae) et de Glycera convoluta (Keferstein) (Glyceridae) Annélides Polychètes Errantes. Ann. Histochim. (sous presse).

  • Monné, L.: On the external cuticle of various helminthes and their role in the host-parasit relationship. A histochemical study. Ark. Zool. 12, 343–358 (1959).

    Google Scholar 

  • Mowry, W.: Observations on the use of sulfuric acid in ether for the sulfation of hydroxyl groups in tissues sections. J. Histochem. Cytochem. 6, 82–83 (1958).

    Google Scholar 

  • —, and Ch. H. Winkler: The coloration of acidic carbohydrates of bacteria and fungi in tissue sections with special reference to capsules of Cryptococcus neoformans, Pneumococci and Staphylococci. Amer. J. Path. 36, 628–629 (1956).

    Google Scholar 

  • Pearse, A. G. E.: Histochemistry — Theoretical and applied. London: Churchill, Ldt. 1954.

    Google Scholar 

  • Pilato, G.: Osservazioni sulla ultrastruttura della cuticola dei policheti Nereidi. Bull. Acad. Gioc. Sci. Nat. Catania, Ser. IV 8, 212–220 (1964).

    Google Scholar 

  • Quintarelli, G., S. Tsuiki, Y. Hashimoto, and W. Pigman: Studies of sialic acidcontaining mucins in Bovine submaxillary and rat sublingual glands. J. Histochem. Cytochem. 9, 176–184 (1961).

    Google Scholar 

  • Randall, J. T.: Aspects of macromolecular orientation in collagenous tissues. Symp. Biocolloids, 113–127 (1956).

  • Reed, R., and K. M. Rudall: Electron microscope studies on the structure of earthworm cuticles. Biochim. biophys. Acta (Amst.) 2, 7–18 (1948).

    Google Scholar 

  • Reynolds, E. S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17, 208–213 (1963).

    Google Scholar 

  • Ritter, B. A., and J. J. Oleson: Combined histochemical staining of acid polysaccharides and 1.2 glycol groupings in paraffin sections of rat tissues. Amer. J. Path. 26, 639–644 (1950).

    Google Scholar 

  • Rudall, K. M.: The distribution of collagen and chitin. Symp. Soc. exp. Biol. 9, 50–70 (1955).

    Google Scholar 

  • Ruska, C., u. H. Ruska: Die Cuticula der Epidermis des Regenwurms (Lumbricus terrestris L.). Z. Zellforsch. 53, 759–764 (1961).

    Google Scholar 

  • Shapiro, J. E.: The structure and development of the cuticulum of digenetic trematodes. Diss. Abstr. 21, 3339 (1957).

    Google Scholar 

  • Singleton, L.: The chemical structure of earthworm cuticle Biochim. Biophys. Acta (Amst.) 24, 67–72 (1957).

    Google Scholar 

  • Slais, J., and Z. Zdarska: Contribution to the histochemistry of the cuticle and cuticular structures of Trematodes. Fol. paras. (Praha) 14, 311–319 (1967).

    Google Scholar 

  • Spicer, S. S.: A correlative study of the histochemical properties of rodent acid mucopolysaccharides. J. Histochem. Cytochem. 8, 18–35 (1960).

    Google Scholar 

  • —: Histochemical differentiation of mammalian mucopolysaccharides. Ann. N.Y. Acad. Sci. 106, 379–387 (1963).

    Google Scholar 

  • —, and D. B. Meyer: Histochemical differentiation of acid mucopolysaccharides by means of combined aldehyde fuchsin-alcian blue staining. Amer. J. clin. Path. 33, 453–460 (1960).

    Google Scholar 

  • —, and L. Warren: Histochemistry of sialic acid containing mucoproteins. J. Histochem. Cytochem. 8, 135–137 (1960).

    Google Scholar 

  • Sundara Rajulu, G., and K. R. Krishnan: On the possible occurence of hyaluronic acid in the cuticle of the earthworm, Megascolex mauritii. Indian J. exp. Biol. 5, 55–66 (1967).

    Google Scholar 

  • Warren, L., and S. S. Spicer: Biochemical and histochemical identification of sialic acid containing mucus of rodent vagina and salivary glands. J. Histochem. Cytochem. 9, 400–408 (1960).

    Google Scholar 

  • Watson, M. R.: The chemical composition of earthworm cuticle. Biochem. J. 68, 416–420 (1958).

    Google Scholar 

  • —, and R. H. Smith: The chemical composition of earthworm cuticle. Biochem. J. 64, p. 10 (1956).

    Google Scholar 

  • Yamada, E.: The fine structure of the gall bladder epithelium of the mouse. J. biophys. biochem. Cytol. 1, 445–457 (1955).

    Google Scholar 

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  1. Laboratoire de Cytologie, 7 Quai Saint-Bernard, Paris

    Catherine Michel

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Michel, C. Ultrastructure et histochimie de la cuticule pharyngienne chez Eulalia viridis Müller, (Annélide Polychète Errante, Phyllodocidae). Z. Zellforsch. 98, 54–73 (1969). https://doi.org/10.1007/BF00344507

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