Skip to main content
SpringerLink
Log in

Die Ultrastruktur der Mitosekerne in den Plasmodien von Physarum polycephalum

The ultrastructure of mitotic nuclei in plasmodia of Physarum polycephalum

  • Published:
Zeitschrift für Zellforschung und Mikroskopische Anatomie Aims and scope Submit manuscript

Summary

The ultrastructure of mitotic nuclei of Physarum polycephalum was investigated by freeze-etching and sectioning techniques.

The nuclear envelope remains intact until telophase, and then dissapears first in the polar regions and later in the interzone. Vesicles covered with ribosomes accumulate in the resulting membrane-free areas, and contribute, together with portions of the old nuclear envelope, to the building of the new nuclear envelope. The nuclear pore complexes remain intact during mitosis.

The mitotic apparatus in metaphase contains continuous microtubules connecting the two poles, and kinetochore-microtubules connecting chromosomes and poles. The disc-shaped kinetochores, 1500 to 2500 Å in diameter, are in contact with one or two microtubules.

In anaphase the mitotic apparatus elongates markedly and the distance between chromosomes and poles shortens slightly. Since the continuous microtubules diverge in the polar regions, they are probably not directly involved in the elongation of the mitotic apparatus. Invaginations of the nuclear envelope indicate that the anaphase separation of chromosomes is accompanied by waves of contractions.

Filaments, 30 to 90 Å in diameter, were observed in the mitotic apparatus. Their arrangement and their similarity with cytoplasmic filaments suggest that they are contractile.

Zusammenfassung

Die Ultrastruktur der mitotischen Kerne in den Plasmodien von Physarum polycephalum wurde mit der Dünnschnitt- und der Gefrierätztechnik untersucht.

Die Kernhülle bleibt während der Mitose bis in die Telophase erhalten, löst sich dann aber zuerst in der Polgegend und später in der Interzone auf. Bläschen, welche mit Ribosomen besetzt sind, lagern sich an die membranfreien Stellen an und bilden, zusammen mit Teilen der alten Kernhülle, die Hülle der Tochterkerne. Die Porenkomplexe bleiben während der Mitose erhalten.

Der Spindelapparat ist in der Metaphase aufgebaut aus durchgehenden Mikrotubuli, welche die Pole verbinden, und aus Kinetochor-Mikrotubuli, welche Chromosomen und Pol verbinden. Die scheibenförmigen Kinetochore 1500–2500 Å im Durchmesser, sind mit einem oder zwei Mikrotubuli verbunden.

In der Anaphase erfolgt eine deutliche Streckung des Spindelapparates und eine geringe Verkürzung des Abstandes zwischen Chromosomen und Pol. Da die durchgehenden Mikrotubuli in der Telophase in der Polgegend divergieren, sind sie kaum direkt (durch Stoßen) an der Verlängerung des Spindelapparates beteiligt. Invaginationen der Kernhülle stimmen mit der Hypothese überein, daß während der Anaphasentrennung der Chromosomen Kontraktionswellen auftreten.

Filamente, 30–90 Å im Durchmesser, wurden im Spindelapparat beobachtet. Ihre Anordnung und die Ähnlichkeit mit den cytoplasmatischen Filamenten von Physarum lassen vermuten, daß es sich um kontraktile Elemente handelt.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literatur

  • Abelson, H. T., Smith, G. H.: The pore annulus relationship in thin section. J. Ultrastruct. Res. 30, 558–588 (1970).

    Article  CAS  PubMed  Google Scholar 

  • Achterrath, M.: Hemmung der Entstehung des Fibrillenmusters von Physarum polycephalum durch Salyrgan. Cytobiologie 1, 159–168 (1969).

    Google Scholar 

  • Adelman, M. R., Borisy, G. G., Shelansky, M. L., Weisenberg, R. C., Taylor, E. W.: Cytoplasmic filaments and microtubules. Fed. Proc. 27, 1186–1193 (1968).

    CAS  PubMed  Google Scholar 

  • Aldrich, H. C.: The ultrastructure of mitosis in myxamoebae and plasmodia of Physarum flavicomum. Amer. J. Bot. 56, 290–299 (1969).

    Article  CAS  Google Scholar 

  • Arnold, J. M.: Cleavage furrow formation in a telolecithal egg (Loligo pealii). I. Filaments in early furrow formation. J. Cell Biol. 41, 894–904 (1969).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Bajer, A.: Chromosome movement and fine structure of the mitotic spindle. In: Aspects of cell motility, p. 285–310 (P. L. Miller, ed.). Cambridge: University Press 1968.

    Google Scholar 

  • —, Molè-Bajer, J.: Formation of spindle fibers, kinetochore orientation, and behaviour of the nuclear envelope during mitosis in endosperm. Fine structural and in vitro studies. Chromosoma (Berl.) 27, 448–484 (1969).

    Article  Google Scholar 

  • Behnke, O., Zelander, T.: Filamentous substructure of microtubules of the marginal bundle of mammalian blood platelets. J. Ultrastruct. Res, 19, 147–165 (1967).

    Article  CAS  PubMed  Google Scholar 

  • Bělař, K.: Beiträge zur Kausalanalyse der Mitose. II. Untersuchungen an den Spermatocyten von Chorthippus (Stenobothrus lineatus) Panz. Wilhelm Roux'Arch. Entwickl.-Mech. Org. 118, 359–484 (1929a).

    Article  Google Scholar 

  • —: Beiträge zur Kausalanalyse der Mitose. III. Untersuchungen an den Staubfadenhaarzellen und Blattmeristemzellen von Tradescantia virginica. Z. Zellforsch. 10, 73–134 (1929b).

    Article  Google Scholar 

  • Brinkley, B. R., Stubblefield, E., Hsu, T. C.: The effects of Colcemid inhibition and reversal on the fine structure of the mitotic apparatus of Chinese Hamster cells in vitro. J. Ultrastruct. Res. 19, 1–18 (1967).

    Article  CAS  PubMed  Google Scholar 

  • Buck, R. C.: Mitosis and meiosis in Rhodnius prolixus: Structure of the spindle and diffuse kinetochore. J. Ultrastruct. Res. 18, 489–501 (1967).

    Article  CAS  PubMed  Google Scholar 

  • Byers, B., Abramson, D. H.: Cytokinesis in HeLa: Post-telophase delay and microtubuleassociated motility. Protoplasma (Wien) 66, 413–435 (1968).

    Article  CAS  Google Scholar 

  • Cohen, A. L.: The “late-formers” — changing concepts of the opsimorphs (Myxomycetes, Acrasieae, Myxobacteria). Arch. Mikrobiol. 59, 59–71 (1967).

    Article  CAS  PubMed  Google Scholar 

  • Cummins, J. E.: Nuclear DNA replication and transcription during the cell cycle of Physarum. In: The cell cycle — gene-enzyme interactions, p. 141–158 (G. Padilla, G. Whitson, and I. Cameron, eds). New York and London: Academic Press 1969.

    Google Scholar 

  • Daniel, J. W., Baldwin, H. H.: Methods of culture for plasmodial myxomycetes. In: Methods of culture for plasmodial myxomycetes. In: Methods in cell physiology, vol. 1, p. 9–41 (D. M. Prescott, ed.) New York and London: Academic Press 1964.

    Google Scholar 

  • —, Kelley, J., Rusch, H. P.: Hematin-requiring plasmodial myxomycete. J. Bact. 84, 1104–1110 (1962).

    CAS  PubMed  PubMed Central  Google Scholar 

  • De Santo, R.S., Dudley, P.L.: Ultramicroscopic filaments in the ascidian Botryllus schlosseri (Pallas) and their possible role in ampullar contractions. J. Ultrastruct. Res. 28, 259–274 (1969).

    Article  PubMed  Google Scholar 

  • Dietz, R.: Bau und Funktion des Spindelapparates. Naturwissenschaften 56, 237–248 (1969).

    Article  CAS  PubMed  Google Scholar 

  • Fahimi, D., Drochmans, P.: Essais de standardisation de la fixation au glutaraldéhyde. I. Purification et détermination de la concentration du glutaraldéhyde. J. Microscopie 4, 725–736 (1965).

    CAS  Google Scholar 

  • Forer, A.: Characterization of the mitotic traction system, and evidence that birefringent spindle fibers neither produce nor transmit force for chromosome movement. Chromosoma (Berl.) 19, 44–98 (1966).

    Article  CAS  Google Scholar 

  • —: Chromosome movements. In: Handbook of molecular cytology, p. 554–601 (A. Lima-de-Faria, ed.). Amsterdam and London: North Holland Publishing Company 1969.

    Google Scholar 

  • Franke, W. W., Scheer, U.: The ultrastructure of the nuclear envelope of amphibian oocytes: A reinvestigation. I. The mature oocyte. J. Ultrastruct. Res. 30, 288–316 (1970).

    Article  CAS  PubMed  Google Scholar 

  • Friend, D. S., Farquhar, M. G.: Functions of coated vesicles during protein absorption in the vas deferens. J. Cell Biol. 35, 357–376 (1967).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Girbardt, M.: Ultrastructure and dynamics of the moving nucleus. In: Aspects of cell motility, p. 249–259 (P. L. Miller, ed.). Cambridge: At the University Press 1968.

    Google Scholar 

  • Goodman, E. M., Ritter, H.: Plasmodial mitosis in Physarum polycephalum. Arch. Protistenk. 111, 161–169 (1969).

    Google Scholar 

  • Guttes, E., Guttes, S.: Mitotic synchrony in the plasmodia of Physarum polycephalum and mitotic synchronization by coalescence of microplasmodia. In: Methods in cell physiology, vol. 1, p. 43–54 (D. M. Prescott, ed.). New York and London: Academic Press 1964.

    Google Scholar 

  • Guttes, S., Guttes, E., Ellis, R. A.: Electron microscope study of mitosis in Physarum polycephalum. J. Ultrastruct. Res. 22, 508–529 (1968).

    Article  CAS  PubMed  Google Scholar 

  • Harris, P.: The effect of mercaptoethanol on the mitotic spindle. Electron microscopy, vol. II, NN-1, 5th Int. Congr. Electr. Micr. Philadelphia (S.S. Breese Jr., ed.). New York and London: Academic Press 1962.

    Google Scholar 

  • —: Some structural and functional aspects of the mitotic apparatus in sea urchin embryos. J. Cell Biol. 14, 475–487 (1962).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • —: Cortical fibers in fertilized eggs of the sea urchin Strongylocentrotus purpuratus. Exp. Cell Res. 52, 677–681 (1968).

    Article  CAS  PubMed  Google Scholar 

  • —, Bajer, A.: Fine structure studies on mitosis in endosperm metaphase of Haemanthus Katherinae bak. Chromosoma (Berl.) 16, 624–636 (1965).

    Article  Google Scholar 

  • Hatano, S., Oosawa, F.: Isolation and characterization of plasmodium actin. Biochim. biophys. Acta (Amst.) 127, 488–498 (1966).

    Article  CAS  Google Scholar 

  • —, Tazawa, M.: Isolation, purification and characterization of myosin B from myxomycete plasmodium. Biochim. biophys. Acta (Amst.) 154, 507–519 (1968).

    Article  CAS  Google Scholar 

  • Holberton, D.: Microtubules in the cytoplasm of an ameba. Nature (Lond.) 222, 680 (1969).

    Article  CAS  Google Scholar 

  • Hollande, A., Cachon, J., Cachon, M.: La dinomitose atractophorienne à Fuseau endonucléaire chez les Radiolaires Thalanophysidae. Son homologie avec la mitose des Foraminifères et avec celle des levures. C. R. Acad. Sci. (Paris) 269, 179–182 (1969).

    Google Scholar 

  • —, Valentin, J.: Infrastructure des axopodes et organisation générale de Sticholonche zanclea Hertwig (Radiolaire, Sticholonchidae). Protistologica 3, 155–166 (1967).

    Google Scholar 

  • —, Valentin, J.: Infrastructure des centromeres et déroulement de la pleuromitose chez les Hypermastigines. C. R. Acad. Sci. (Paris) 266, 367–370 (1968).

    Google Scholar 

  • Hughes, A. F. W.: The mitotic cycle. New York and London: Academic Press 1952.

    Google Scholar 

  • Ichida, A. A., Fuller, M. S.: Ultrastructure of mitosis in the aquatic fungus Catenaria anguillulae. Mycologia 60, 141–155 (1968).

    Article  CAS  PubMed  Google Scholar 

  • Inoué, S.: Organization and function of the mitotic spindle. In: Primitive motile systems in cell biology, p. 549–598 (R. D. Allen and N. Kamiya, ed.). New York and London: Academic Press 1964.

    Chapter  Google Scholar 

  • —, Bajer, A.: Birefringence in endosperm mitosis. Chromosoma (Berl.) 12, 48–63 (1961).

    Article  Google Scholar 

  • Ishikawa, H., Bischoff, R., Holtzer, H.: Formation of arrowhead complexes with heavy meromyosin in a variety of cell types. J. Cell Biol. 43, 312–328 (1969).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Jenkins, R. A.: Fine structure of division in ciliate protozoa. J. Cell Biol. 34, 463–481 (1967).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Jensen, C., Bajer, A.: Effects of dehydration on the microtubules of the mitotic spindle. J. Ultrastruct. Res. 26, 367–386 (1969).

    Article  CAS  PubMed  Google Scholar 

  • Johnson, U. G., Porter, K. R.: Fine structure of cell division in Chlamydomonas Reinhardi Basal bodies and microtubules. J. Cell Biol. 38, 403–425 (1968).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Jokelainen, P. T.: The ultrastructure and spatial organization of the metaphase kinetochore in mitotic rat cells. J. Ultrastruct. Res. 19, 19–44 (1967).

    Article  CAS  PubMed  Google Scholar 

  • Kamiya, N.: The mechanism of cytoplasmic movement in a myxomycete plasmodium. In: Aspects of cell motility, p. 199–214 (P. L. Miller, ed.). Cambridge: At the University Press 1968.

    Google Scholar 

  • Karnovsky, M.: A formaldehyde — glutaraldehyde fixative of high osmolarity for use in electron microscopy. J. Cell Biol. 27, 137A (1965).

    Google Scholar 

  • Korn, E. D., Greenblatt, C. L., Lees, A. M.: Synthesis of unsaturated fatty acids in the slime mold Physarum polycephalum and the zooflagellates Leishmania tarantolae, Trypanosoma lewisii, and Crithidia spec.: A comparative study. J. Lipid Res. 6, 43–50 (1965).

    CAS  PubMed  Google Scholar 

  • Kubai, D. F., Ris, H.: Division in the dinoflagellate Gyrodinium Cohnii (Schiller): A new type of nuclear reproduction. J. Cell Biol. 40, 508–528 (1969).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kudo, R. R.: Protozoology, 4th ed. Springfield, Ill. C. C. Thomas, Publ. 1954.

    Google Scholar 

  • Lessie, P. E., Lovett, J. S.: Ultrastructural changes during sporangium formation and zoospore differentiation in Blastocladiella emersonii. Amer. J. Bot. 55, 220–236 (1968).

    Article  CAS  Google Scholar 

  • Luykx, P.: The structure of the kinetochore in meiosis and mitosis in Urechis eggs. Exp. Cell Res. 39, 643–657 (1965).

    Article  CAS  PubMed  Google Scholar 

  • Marsland, D., Landau, J. V.: The mechanisms of cytokinesis: Temperature-pressure studies on the cortical gel systems in various eggs. J. Exp. Zool. 125, 507–539 (1954).

    Article  Google Scholar 

  • Mazia, D.: Mitosis and the physiology of cell division. In: The cell (Biochemistry, physiology, morphology) vol. III, p. 77–412 (J. Brachet and A. E. Mirsky, eds.). New York and London: Academic Press 1961.

    Google Scholar 

  • —, Zimmermann, A. M.: SH-compounds in mitosis. The effect of mercaptoethanol on the structure of the mitotic apparatus in sea urchin eggs. Exp. Cell Res. 15, 138–153 (1958).

    Article  CAS  PubMed  Google Scholar 

  • McIntosh, J. R., Hepler, P. K., vanWie, D. G.: Model for mitosis. Nature (Lond.) 224, 659–663 (1969).

    Article  Google Scholar 

  • Mollenhauer, H. H.: Plastic embedding mixtures for use in electron microscopy. Stain Technol. 39, 111–114 (1963).

    Google Scholar 

  • Moor, H., Mühlethaler, K.: Fine structure in frozen-etched yeast cells. J. Cell Biol. 17, 609–628 (1963).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Nakajima, H., Allen, R. D.: The changing pattern of birefringence in plasmodia of the slime mold Physarum polycephalum. J. Cell Biol. 25, 361–374 (1965).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Nicklas, R. B., Staehly, C. A.: Chromosome micromanipulation. I. The mechanics of chromosome attachment to the spindle. Chromosoma (Berl.) 21, 1–16 (1967).

    Article  CAS  Google Scholar 

  • Oestergren, G., Molè-Bajer, J., Bajer, A.: An interpretation of transport phenomena at mitosis. Ann. N. Y. Acad. Sci. 90, 381–408 (1960).

    Article  Google Scholar 

  • Pickett-Heaps, J. D., Fowke, L. C.: Cell division in Oedogonium. I. Mitosis, cytokinesis, and cell elongation. Aust. J. biol. Sci. 22, 857–894 (1969).

    Google Scholar 

  • Porter, K. R.: Cytoplasmic microtubules and their functions. In: Principles of biomolecular organization (A Ciba foundation symposium), p. 308–356 (G. E. W. Wolstenholme and M. O'Connor, eds.). London: Churchill Ltd. 1966.

    Google Scholar 

  • —, Machado, R. D.: Studies on the endoplasmic reticulum. IV. Its form and distribution during mitosis in cells of onion root tips. J. biophys. biochem. Cytol. 7a, 167–180 (1960).

    Article  Google Scholar 

  • Reger, J. F., Florendo, N. T.: Studies on motile, non tubule-containig, filiform spermatozoa of the ostracod Cypridopsis. II. Mature spermatozoa. J. Ultrastruct. Res. 28, 250–258 (1969)

    Article  Google Scholar 

  • Reynolds, E. S.: The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J. Cell Biol. 17, 208–213 (1963).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Robbins, E., Jentzsch, G.: Ultrastructural changes in the mitotic apparatus at the metaphase to anaphase transition. J. Cell Biol. 40, 678–691 (1969).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Robinow, C. F., Marak, J.: A fiber apparatus in the nucleus of the yeast cell. J. Cell Biol. 29, 129–152 (1966).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Ross, I. K.: Chromosome numbers in pure and gross cultures of myxomycetes. Amer. J. Bot. 53, 712–718 (1966).

    Article  Google Scholar 

  • —: Nuclear membrane behaviour during mitosis in normal and heteroploid myxomycetes. Protoplasma (Wien) 66, 173–184 (1968).

    Article  CAS  Google Scholar 

  • Roth, L. E.: Electron microscopy of mitosis in amebae. III. Cold and urea treatments: A basis for tests of direct effects of mitotic inhibitors on microtubule formation. J. Cell Biol. 34, 47–59 (1967).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • —, Daniels, E. W.: Electron microscopic studies of mitosis in amebae. II. The giant ameba Pelomyxa carolinensis. J. Cell Biol. 12, 57–78 (1962).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • —, Pihlaja, D. J., Shigenaka, Y.: Microtubules in the heliozoan axopodium. I. The gradion hypothesis of allosterism in structural proteins. J. Ultrastruct. Res. 30, 7–37 (1970).

    Article  CAS  PubMed  Google Scholar 

  • Roth, T. F., Porter, K. R.: Specialized sites on the cell surface for protein uptake. In: Electron microscopy, vol. II, LL-4 (S. S. Breese, Jr., ed.). 5th Int. Congr. Electr. Micr. Philadelphia. New York and London: Academic Press 1962.

    Google Scholar 

  • —: Yolk protein uptake in the mosquito Aedes aegyptii L. J. Cell Biol. 20, 313–332 (1964).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Rusch, H. P.: Some biochemical events in the life cycle of Physarum polycephalum. In: Advances in cell biology, vol. 1, 297–328, (D. M. Prescott, ed.) New York: Appleton-century-Crofts (1969a).

    Google Scholar 

  • —: Some biochemical events in the growth cycles of Physarum polycephalum. Fed. Proc. 28, 1761–1770 (1969b).

    CAS  PubMed  Google Scholar 

  • —, Sachsenmaier, W., Behrens, K., Gruter, V.: Synchronization of mitosis by the fusion of the plasmodia of Physarum polycephalum. J. Cell Biol. 31, 204–209 (1966).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Schroeder, T. E.: Cytokinesis: Filaments in the cleavage furrow. Exp. Cell Res. 53, 272–276 (1968).

    Article  CAS  PubMed  Google Scholar 

  • Soyer, M. O.: Rapport existant entre chromosomes et membrane nucléaire chez un Dinoflagellé parasite du genre Blastodinium Chatton. C. R. Acad. Sci. (Paris) 268, 2082–2084 (1969).

    Google Scholar 

  • Swann, M. M.: Protoplasmic structure and mitosis. II. The nature and cause of birefringence changes in the sea urchin egg at anaphase. J. exp. Biol. 28, 434–444 (1951).

    Google Scholar 

  • Szollosi, D.: Cortical cytoplasmic filaments of cleaving eggs: A structural element corresponding to the contractile ring. J. Cell Biol. 44, 193–209 (1970).

    Article  Google Scholar 

  • Tilney, L. G.: The assembly of microtubules and their role in the development of cell form. Develop. Biol., Suppl. 2, 63–102 (1968).

    Google Scholar 

  • —, Byers, B.: Studies on the microtubules in heliozoa. V. Factors controlling the organization of microtubules in the axonemal pattern in Echinosphaerium (Actinosphaerium) nuleofilum. J. Cell Biol. 43, 148–165 (1969).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • —, Marsland, D.: A fine structural analysis of cleavage induction and furrowing in the eggs of Arbacia punctulata. J. Cell Biol. 42, 170–184 (1969).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Vivier, E.: Observations ultrastructurales sur l'envelope nucléaire et ses “pores” chez les Sporozoaires. J. Microscopie 6, 371–390 (1967).

    Google Scholar 

  • Wilson, H. J.: The fine structure of the kinetochore in meiotic cells of Tradescantia. Planta (Berl.) 78, 379–385 (1968).

    Article  CAS  Google Scholar 

  • —: Arms and bridges on microtubules in the mitotic apparatus. J. Cell Biol. 40, 854–859 (1969).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wohlfarth-Bottermann, K. E.: Differentiations of the ground cytoplasm and their significance for the generation of the motive force of ameboid movement. In: Primitive systems in cell biology, p. 79–109 (R. D. Allen and N. Kamiya, eds). New York and London: Academic Press 1964.

    Chapter  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratorium für Elektronenmikroskopie Institut für Allgemeine Botanik Eidgenössische Technische Hochschule Zürich, Schweiz

    Ulrich Ryser

  2. Expérimentales sur le cancer, Institut Suisse de recherches, Bugnon 21, 1005, Lausanne, Schweiz

    Ulrich Ryser

Authors
  1. Ulrich Ryser
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Die vorliegende Arbeit wurde der Eidgenössischen Technischen Hochschule in Zürich als Dissertation vorgelegt.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ryser, U. Die Ultrastruktur der Mitosekerne in den Plasmodien von Physarum polycephalum . Z. Zellforsch. 110, 108–130 (1970). https://doi.org/10.1007/BF00343989

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00343989

Key-Words

Search

Navigation