Abstract
Cell-free cytoplasm isolated from meiotic Xenopus egg extracts reconstitutes microtubule phenomena in vitro. These crude extracts assemble bipolar meiotic spindles and are readily fractionated for biochemical assays, providing a good tool to dissect molecular mechanism. We developed techniques for immunoelectron microscopy of microtubule structures assembled in perfusion chambers and in solution.
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Acknowledgments
Our work was funded primarily by NIH grant GM23928.
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Coughlin, M., Groen, A.C., Mitchison, T.J. (2014). Electron Microscopy of Microtubule Cytoskeleton Assembly In Vitro. In: Kuo, J. (eds) Electron Microscopy. Methods in Molecular Biology, vol 1117. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-776-1_12
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DOI: https://doi.org/10.1007/978-1-62703-776-1_12
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Publisher Name: Humana Press, Totowa, NJ
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