Skip to main content
SpringerLink
Log in

Über das Wachstum der Pankreaszellen von Säugetieren als Monolayer Cultures

I. Züchtungsmethode, Morphologie und Insulingehalt

Monolayer cultures of pancreatic tissue

I. Methods of culture, morphology, insulin content of tissues and culture medium

  • Published:
Zeitschrift für Zellforschung und Mikroskopische Anatomie Aims and scope Submit manuscript

Summary

Monolayer cultures of large fibroblastoid cells could be cultivated from cell suspensions of pancreatic tissue of juvenile rats and guinea pigs as well as human fetuses. These cells are stained with aldehyde fuchsin exactly like the B-cells of the islets of Langerhans; with pseudo-isocyanine their reaction is positive to insulin. It is possible by means of an immunological method to determine “insulin” (=IRI=immuno reactive insulin) in the collected nutrient medium as well as in the cells after formation a complete monolayer.

Zusammenfassung

Aus Zellsuspensionen von Pankreasgewebe jugendlicher Ratten und Meerschweinchen sowie menschlicher Feten züchteten wir Monolayer Cultures großer fibroblastoider Zellen. Diese färben sich mit Aldehydfuchsin ebenso wie B-Zellen der Langerhansschen Inseln an; mit Pseudoisocyanin geben sie eine positive Reaktion auf Insulin. Mit Hilfe einer immunologischen Methode konnten wir sowohl in der gesammelten Nährlösung als auch in den Zellen nach Ausbildung eines Zellrasens „Insulin“ (=IRI=immuno reactive insulin) feststellen.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literatur

  • Altmann, H. W.: Über den Funktionsformwechsel des Kernes im exokrinen Gewebe des Pankreas. Z. Krebsforsch. 58, 632–645 (1952).

    Google Scholar 

  • Anderson, A., C. Hellerström, and B. Petersson: Phase-Contrast microscopy of fresh and cultured pancreatic islet cells of Guinea-pigs. Z. Zellforsch. 82, 110–117 (1967).

    Google Scholar 

  • Ferner, H.: Das Inselsystem des Pankreas. Stuttgart: Georg Thieme 1952.

    Google Scholar 

  • Golosow, N., and C. Grobstein: Epitheliomesenchymal interaction in pancreatic morphogenesis. Develop. Biol. 4, 242–255 (1962).

    Google Scholar 

  • Hsu, T. C., and T. Y. Lou: Nuclear extrusion in cells of Cloudman melanoma in vitro. In: Pigment cell biol. (Hrsg. M. Gordon), S. 315–325. New York: Academic Press Inc. 1959.

    Google Scholar 

  • Jönsson, L.-E., J. Pontén, and J. Thorell: Long-term production of insulin by adult rat pancreas in vitro. Diabetologia (Abstracts) 2, 234 (1966).

    Google Scholar 

  • Kerp, L., S. Steinhilber u. H. Kasemir: Ein Verfahren zum Nachweis insulinbildender Antikörper durch Differentialadsorption. Klin. Wschr. 44, 560–567 (1966).

    Google Scholar 

  • McManus, J. F. A., and R. W. Mowry: Staining methods. Histologic and histochemical. New York: Paul B. Hoeber Inc. 1960.

    Google Scholar 

  • Meade, R. C., and H. M. Klitgaard: A simple method for immunoassay of human serum insulin. J. nucl. Med. 3, 407 (1962).

    Google Scholar 

  • Moskalewski, S.: Isolation and culture of the islets of Langerhans of the Guinea pig. Gen. comp. Endocr. 5, 342–353 (1965).

    Google Scholar 

  • Murrell, L. R.: Mammalian pancreatic islet tissue in organ culture. I. Methods of culture and in vitro histogenesis. Exp. Cell Res. 41, 350–364 (1966).

    Google Scholar 

  • —, C. R. Morgan, and A. Lazarow: Mammalian pancreatic islet tissue in organ culture. II. Insulin contents of tissues and culture medium. Exp. Cell Res. 41, 365–375 (1966).

    Google Scholar 

  • Schiebler, T. H., u. S. Schiessler: Über den Nachweis von Insulin mit den metachromatisch reagierenden Pseudoisocyaninen. Histochemie 1, 445–465 (1959).

    Google Scholar 

  • Steiner, D. F., and P. E. Oyer: The biosynthesis of insulin and a probable precursor of insulin by a human islet cell adenoma. Proc. nat. Acad. Sci. (Wash.) 57, 473–480 (1967a).

    Google Scholar 

  • —, D. Cunningham, L. Spigelman, and B. Aten: Insulin biosynthesis: Evidence for a precursor. Science 157, 697–700 (1967b).

    Google Scholar 

  • Takaki, R., M. N. Goldstein, and L. J. Journey: Growth and functional characteristics of cell lines from pancreas of adult rabbits. Excerpta Med. (Amst.) Sect. I 18, T 91 (1964).

    Google Scholar 

  • Vecchio, D., A. Luyckx, G. R. Zahnd, and A. E. Renold: Insulin release induced by glucagon in organ cultures of fetal rat pancreas. Metabol. Clin. Exp. 15, 577–581 (1966).

    Google Scholar 

  • Zagury, D.: La culture de pancréas endocrine chez le Rat et l'insulogénèse. C. R. Acad. Sci. (Paris) 249, 2834–2835 (1959).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Farbwerke Hoechst AG, Deutschland

    I. Hilwig, S. Schuster, W. Heptner & E. v. Wasielewski

Authors
  1. I. Hilwig
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. Schuster
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. W. Heptner
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. E. v. Wasielewski
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hilwig, I., Schuster, S., Heptner, W. et al. Über das Wachstum der Pankreaszellen von Säugetieren als Monolayer Cultures. Zeitschrift für Zellforschung 90, 333–346 (1968). https://doi.org/10.1007/BF00341991

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00341991

Search

Navigation