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Isolation and Culture of Human Dermal Fibroblasts

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Skin Tissue Engineering

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1993))

Abstract

Dermal fibroblasts are the main cell type present in skin connective tissue (dermis). Fibroblasts interact with epidermal cells during hair development and in interfollicular skin. Moreover, they play an essential role during cutaneous wound healing and in bioengineering of skin. Hence, culture of primary fibroblast is gaining in importance. In addition, fibroblasts established from skin biopsies provide a powerful tool for investigating normal skin physiology or specific disease states. In this chapter, detailed procedures for establishing and maintaining primary cultures of adult human dermal fibroblasts are described.

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Author information

Authors and Affiliations

  1. Occupational and Environmental Medicine, Medical Sciences, Uppsala University Hospital, Uppsala, Sweden

    Marta A. Kisiel

  2. Tissue Biology Research Unit, University Children’s Hospital Zurich, University of Zurich, Zurich, Switzerland

    Agnes S. Klar

  3. Children’s Research Center, University Children’s Hospital Zurich, Zurich, Switzerland

    Agnes S. Klar

Authors
  1. Marta A. Kisiel
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  2. Agnes S. Klar
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Corresponding author

Correspondence to Agnes S. Klar .

Editor information

Editors and Affiliations

  1. University Children’s Hospital Zurich, Zurich, Switzerland

    Sophie Böttcher-Haberzeth  & Thomas Biedermann  & 

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Kisiel, M.A., Klar, A.S. (2019). Isolation and Culture of Human Dermal Fibroblasts. In: Böttcher-Haberzeth, S., Biedermann, T. (eds) Skin Tissue Engineering. Methods in Molecular Biology, vol 1993. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9473-1_6

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  • DOI: https://doi.org/10.1007/978-1-4939-9473-1_6

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9472-4

  • Online ISBN: 978-1-4939-9473-1

  • eBook Packages: Springer Protocols

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