Abstract
Frequencies and amplitudes of miniature endplate potentials (MEPP's) were recorded from neuromuscular junctions of the rat phrenic nerve-diaphragm preparation in vitro. Superfusion of the preparations with Ringer solution containing thallium acetate (Tlac) gradually increased the frequency of MEPP's by a factor of 10 within 30 min (1×10−3 mol/l Tlac) and 180 min (5×10−4 mol/l Tlac), whereas the amplitude of MEPP's remained unchanged. Frequency of MEPP's fitted a Poisson-distribution which persisted during superfusion with Tl+-Ringer. Sub-MEPP amplitudes remained unaffected by the action of thallium. It is concluded that thallium interferes presynaptically with spontaneous transmitter release.
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References
Åkerman KO, Nicholls DG (1983) Physiological and bioenergetic aspects of mitochondrial calcium transport. Rev Physiol Biochem Pharmacol 95: 150–201
Alneas E, Rahamimoff R (1975) On the role of mitochondria in transmitter release from motor nerve terminals. J Physiol 248: 285–306
Barton SB, Cohen IS, van der Kloot W (1983) The calcium dependence of spontaneous and evoked quantal release at the neuromuscular junction. J Physiol 337: 735–751
Britten JS, Blank M (1968) Thallium activation of the (Na+-K+)-activated ATPase of rabbit kidney. Biochim Biophys Acta 159: 160–166
Cavieres JD, Ellory JC (1974) Thallium and the sodium pump in human red cells. J Physiol 242: 243–266
Colmeus C, Gomez S, Molgo J, Thesleff S (1982) Discrepancies between spontaneous and evoked synaptic potentials at normal, regenerating and botulinum toxin poisoned mammalian neuromuscular junctions. Proc R Soc Lond [Biol] 215: 63–74
Cooke JD, Quastel MJ (1973) Transmitter release by mammalian motor nerve terminals in response to focal polarization. J Physiol 228: 377–405
Gage PW, Hubbard JI (1965) Evidence for a poisson distribution of miniature end-plate potentials and some implications. Nature 208: 395–396
Hubbard JI, Jones SF, Landau EM (1968) On the mechanism by which calcium and magnesium affect the spontaneous release of transmitter from mammalian motor nerve terminals. J Physiol 194: 355–380
Hubbard JI, Llinas R, Quastel DMJ (1969) Electrophysiologica analysis of synaptic transmission. Edward Arnold Publ., London. Printed in Great Britain by The Camelot Press Ltd.
Hughes MN, Man WK, Whaler BC (1978) The toxicity of thallium (I) to cardiac and skeletal muscle. Chem Biol Interact 23: 85–97
Kaeser HE (1962) Funktionsprüfungen peripherer Nerven bei experimentellen Polyneuriden und bei der Wallerschen Degeneration. Dtsch Z Nervenheilkd 183: 268–304
Liley AW (1956a) An investigation of spontaneous activity at the neuromuscular junction of the rat. J Physiol 132: 650–666
Liley AW (1956b) The effects of presynaptic polarization on the spontaneous activity at the mammalian neuromuscular junction. J Physiol 134: 427–443
Llinas RR, Heuser JE (1977) Depolarization-release coupling systems in neurons. Neurosci Res Prog Bull 15/4: 557–687
Magleby KL, Miller DC (1981) Is the quantum of transmitter release composed of subunits? A critical analysis in the mouse and frog. J Physiol 311: 267–287
Mambrini J, Benoit RP (1964) Action du calcium sur la jonction neuro-musculaire chez la grenouille. CR Soc Biol 7: 1454–1458
Matteson DR, Kriebel ME, Llados F (1981) A statistical model indicates that miniature end-plate potentials and unitary evoked endplate potentials are composed of subunits. J Theor Biol 90: 337–363
Melnick RL, Monti LG, Motzkin SM (1976) Uncoupling of mitochondrial oxidative phosphorylation by thallium. Biochem Biophys Res Commun 69/1: 68–73
Miller DC, Weinstock MM, Magleby KL (1978) Is the quantum of transmitter release composed of subunits? Nature 274/5669: 388–390
Passarge C, Wieck HH (1965) Thallium-Polyneuritis. Fortschr Neurol Psychiatr 33/9: 478–576
Prick JJ (1979) Thallium poisoning. Handbook of clinical neurology, vol 36, pp 239–278
Rahamimoff R, Erulkar SD, Alneas E, Meiri H, Rotshenker S, Rahamimoff H (1976) Modulation of transmitter release by calcium and nerve impulse. Cold Spring Harbor Symp Quant Biol XL: 107–116
Saris N-EL, Skulskii IA, Savina MV, Glasunov VV (1981) Mechanism of mitochondrial transport of thallous ions. J Bioenerg Biomembr 13: 51–59
Skulskii IA, Manninen V, Järnefelt J (1973) Interaction of thallous ions with the cation transport mechanism in erythrocytes. Biochim Biophys Acta 298: 702–709
Spencer PS, Peterson ER, Madrid RA, Raine CS (1973) Effects of thallium salts on neuronal mitochondria in organotypic cord-ganglia-muscle combination cultures. J Cell Biol 58: 79–95
Steinbach JH, Stevens CF (1976) Neuromuscular transmission. In: Llinas R, Precht W (eds) Frog neurobiology. Springer, Berlin Heidelberg New York, pp 33–77
Wiegand H, Csicsaky M, Krämer U (1984) The action of thallium acetate on neuromuscular transmission in the rat phrenic nerve-diaphragm preparation. Arch Toxicol 55: 55–58
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Wiegand, H., Papadopoulos, R., Csicsaky, M. et al. The action of thallium acetate on spontaneous transmitter release in the rat neuromuscular junction. Arch Toxicol 55, 253–257 (1984). https://doi.org/10.1007/BF00341021
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DOI: https://doi.org/10.1007/BF00341021