Abstract
Tissue zinc and copper concentrations undergo marked changes in the neonatal rat during the first several weeks of life and it was of considerable interest to study the effect of cadmium exposure on these ontogenic changes. Long evans rats received either 2 or 10 μmol cadmium chloride per kg SC at 9 days of age and were sacrificed at 20 or 36 days of age. Tissue copper and zinc concentrations in cadmium-treated rats were compared to those of age-matched controls for statistically significant changes. The tissue affected, the element altered and the direction of change in concentration, increased (+) or decreased (-), are summarized for the two dosing groups (age at dosing, age at sacrifice in days): 2 μmol/kg (9, 20): kidney Zn (+), blood Zn (-), cerebral Cu (-), cerebellar Cu (+); 2 μmol/kg (9, 36): blood Zn (-); 10 μmol/kg (9, 20); liver, kidney, cerebral and cerebellar and blood Zn (-), cerebellar Cu (+); 10 μmol/kg (9, 36]: liver and heart Zn (+), blood Zn (-); liver and heart Zn (+), blood Zn (-); kidney, cerebral, cerebellar and heart Cu (+). Changes in tissue zinc or copper concentrations produced by cadmium treatment could not be accounted for by the direct replacement of these elements by cadmium and may be due to alterations in transport of these elements. These results indicate that early life exposure to low levels of camium can have large and persistent effects on the distribution of the essential metals, copper and zinc.
Similar content being viewed by others
References
Arvidson B (1983a) Cadmium toxicity and neural cell damage. In: Dreosti I, Smith R (eds), Neurobiology of the trace elements, Humana Press, Clifton, New Jersey. Vol 1, pp 57–78
Arvidson B (1983b) Influence of age upon the development of cadmium-induced vascular lesions in rat sensory ganglia. Environ Res 32: 240–246
Bakka A, Samarawickrama GP, Webb M (1981) Metabolism of zinc and copper in the neonate: Effect of cadmium administration during late gestation in the rat on zinc and copper metabolism of the newborn. Chem Biol Interact 34: 161–171
Barr M (1973) The teratogenicity of cadmium chloride in two stocks of Wistar rats. Teratology 7: 237–242
Beach RS, Gershwin ME, Hurley LS (1980) Growth and development in postnatally zinc-deprived rats. J Nutr 110: 201–211
Brady FO (1983) Metabolism of zinc and copper in the neonate: Zinc thionein in developing rat brain, heart, lung, spleen, and thymus. Life Sci 32: 2981–2987
Cahill DF, Bercegeau MS, Haggerty RC, Gerding JE, Gray LE (1980) Age-related retention and distribution of ingested Mn3O4 in the rat. Toxicol Appl Pharmacol 53: 83–91
Chernoff N (1973) Teratogenic effects of cadmium in rats. Teratology 8: 29–32
Dipaola RV, Kanfer JN, Newberne PM (1974) Copper deficiency and central nervous system myelination in the rat: Morphological and biochemical studies. J Neuropathol Exp Neurol 33: 226–236
Feller DJ, O'Dell BL (1980) Dopamine and norepinephrine in discrete areas of the copper-deficient rat brain. J Neurochem 34: 1259–1263
Forbes GB, Reina JC (1972) Effect of age on gastrointestinal absorption (Fe, Sr, Pb) in the rat. J Nutr 102: 647–652
Friberg LT, Piscator M, Nordberg GF, Kjellstrom T (1974) Cadmium in the environment, 2nd edition, C.R.C. Press, Cleveland, Ohio pp 86–87
Gabbiani G, Gregory A, Biac D (1967a) Cadmium induced selective lesions of the sensory ganglia. J Neuropathol Exp Neurol 26: 498–506
Gabbiani G, Biac D, Deziel C (1967b) Toxicity of cadmium for the central nervous system. Exp Neurol 18: 154–160
Goering PL, Klaassen CD (1984) Resistance to cadmium-induced hepatotoxicity in immature rats. Toxicol Appl Pharmacol 74: 321–329
Hastings L, Choudbury H, Petering HG, Cooper GP (1978) Behavioral and biochemical effect of low level prenatal cadmium exposure in rats. Bull Environ Contam Toxicol 20: 96–101
Henke G, Sachs HW, Bohn G (1970) Cadmium. Bestimmungen in Leber and Nieren von Kindern and Jugendlichen durch Neutronenaktivierungsanalyse. Arch Toxikol 26: 8–16
Hurley LS, Swenerton H (1966) Congenital malformations resulting from zinc deficiency in rats. Proc Soc Exp Biol Med 123: 692–697
Irons RD, Smith JC (1976) Prevention by copper of cadmium sequestration by metallothionein in liver. Chem Biol Interact 15: 289–284
Jacobs RM, Lee-Jones AO, Spivey Fox MR, Fry, Jr BE (1978a) Retention of dietary cadmium and the ameliorative effect of zinc, copper and manganese in Japanese quail. J Nutr 108: 22–32
Jacobs RM, Lee-Jones AO, Fry, Jr BE, Spivey Fox MR (1978b) Decreased long term retention of 115mCd in Japanese quail produced by a combined supplement of zinc, copper and manganese. J Nutr 108: 901–910
Jugo S (1977) Metabolism of toxic heavy metals in growing organisms. Environ Res 13: 36–46
Keen CL, Lonnerdahl B, Clegg M, Hurley LS (1981) Developmental changes in composition of rat milk: Trace elements, minerals, protein, carbohydrate and fat. J Nutr 111: 226–236
Keen CL, Lonnerdahl B, Hurley LS (1983) Teratogenic effects of copper deficiency and excess. In: Sorenson JRJ (ed) Inflammatory diseases and copper, Humana Press, Clifton, New Jersey. pp 109–121
Kello D, Kostial K (1977) Influence of age on whole-body retention and distribution of 115mCd in the rat. Environ Res 14: 92–98
Matrone G (1974) Chemical parameters in trace-element antagonisms. In: Hoekstra WG, Suttie JW, Ganther HE, Mertz W (eds) Trace element metabolism in animals — 2, University Park Press, Baltimore, pp 91–103
Miller GJ, Wylie MJ, McKeown D (1976) Cadmium exposure and renal accumulation in an Australian urban population. Med J Austl i: 20–23
Morgan RF, O'Dell BL (1977) Effect of copper deficiency on the concentrations of catecholamines and related enzyme activities in the rat brain. J Neurochem 28: 207–213
Onosaka S, Cherian MG (1981) The induced synthesis of metallothionein in various tissues of rat in response to metals. I. Effect of repeated injection of cadmium salts. Toxicology 22: 91–101
Panemangalore M, Banerjee D, Onosaka S, Cherian MG (1983) Changes in the intracellular accumulation and distribution of metallothionein in rat liver and kidney during postnatal development. Dev Biol 97: 95–102
Panemangalore M, Cherian MG (1983) Metabolism of parenterally administered zinc and cadmium in liver of newborn rats. Chem Biol Interact 45: 327–339
Parizek J (1957) The destructive effect of cadmium ion on testicular tissue and its prevention by zinc. J Endocrinol 15: 56–63
Rastogi RB, Merali Z, Singhal RL (1977) Cadmium alters behaviour and the biosynthetic capacity for catecholamines and serotonin in neonatal rat brain. J Neurochem 28: 789–794
Sandstead HH, Gillespie DD, Brady RN (1972) Zinc deficiency: Effect on brain of the suckling rat. Pediatr Res 6: 119–125
Sandstead HH, Wallwork JC, Halas ES, Tucker DM, Dvergsten CL, Strobel DA (1983) Zinc and central nervous system function. In: Sarkar B (ed). Biological aspects of metals and metalrelated diseases, Raven Press, New York, pp 225–241
Sasser LB, Jarboe GE (1977) Intestinal absorption and retention of cadmium in the neonatal rat. Toxicol Appl Pharmacol 41: 423–431
Schlaepfer WW (1974) Sequential study of endothelial changes in acute cadmium intoxication. Lab Invest 25: 556–564
Schmid BP, Kao J, Goulding E (1985) Evidence for reopening of the cranial neural tube in mouse embryos treated with cadmium chloride. Experientia 41: 271–272
Schulte-Lobbert FJ, Bohn G, Acker L (1978) Untersuchungen zur exogenen Cadmiumaufnahme bei Säuglingen und Kleinkindern. Beitr Gerichtl Med 36: 491–495
Sugawara N, (1977) Influence on cadmium of zinc distribution in the mouse liver and kidney. Toxicol Appl Pharmacol 42: 377–386
Teare FW, Read PR, Pyttel RB, Jasansky PA (1978) Short- and long-term cadmium distribution in rat livers after different routes of administration. Arch Environ Health 33: 53–58
Thomas DJ, Fisher HL, Hall LL, Mushak P (1982) Effects of age and sex on retention of mercury by methyl mercury-treated rats. Toxicol Appl Pharmacol 62: 445–454
Thompson DM, Banerjee D, Cherian MG (1985) Metallothionein synthesis and localization in relation to metal storage in rat liver during gestation. Can J Biochem Cell Biol 63: 16–22
Tsuchiya K, Seki Y, Sugita M (1976) Cadmium concentrations in the organs and tissues of cadavers from accidental death. Keio J Med 25: 83–90
Waalkes MP, Klaassen CD (1984) Postnatal ontogeny of metallothionein in various organs of the rat. Toxicol Appl Pharmacol 74: 314–320
Wong K-L, Klaassen CD (1979) Isolation and characterization of metallothionein which is highly concentrated in newborn rat liver. J Biol Chem 254: 12399–12403
Wong K-L, Cachia R, Klaassen CD (1980) Comparison of the toxicity and tissue distribution of cadmium in newborn and adult rats after repeated administration. Toxicol Appl Pharmacol 56: 317–325
Zimmerman AW, Matthieu J-M, Quarles RM, Brady RO, Hsu JM (1976) Hypomyelination in copper-deficient rats. Arch Neurol 33: 111–119
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Thomas, D.J., Mushak, P. Effects of cadmium exposure on zinc and copper distribution in neonatal rats. Arch Toxicol 58, 130–135 (1986). https://doi.org/10.1007/BF00340971
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00340971