Summary
Twenty-two borrelidin resistant mutants of Saccharomyces cerivisiae were isolated, studied genetically and their threonyl-tRNA-synthetase was investigated. The borrelidin resistant mutants are classified into four groups. In the first group borrelidin resistance is coupled to the gene HOM3 coding for aspartokinase, in the second group to the gene LEU1. The borrelidin resistance in group three and four is not coupled to anyone of the genetic markers tested. Borrelidin resistance exhibited dominant behavior in all mutants except in the mutant of group 4. The properties of the ThrRS of the mutants of group one, two and four was found to be like the ones of the wild types. However the mutants of group three exhibit a structurally altered ThrRS, which is no longer inhibited by borrelidin.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ehresmann, B., Karst, F., Weil, J.H.: Regulation of the biosynthesis of valyl-tRNA-synthetase in yeast. Biochim. biophys. Acta (Amst.) 254, 226–236 (1971)
Fink, F.I.: The biochemical genetics of yeast. In: Methods in enzymology (H. Tabor and C.W. Tabor, eds.), Vol. 17 A, pp. 59–78. London and New York: Academic Press 1970
Grenson, M., Mousset, M., Wiame, J.M., Bechet, J.: Multiplicity of the amino acid permeases in Saccharomyces cerivisiae. I. Evidence for a specific arginine-transporting system. Biochim. biophys. Acta (Amst.) 127, 325–338 (1966)
Hartwell, L.H., McLaughlin, C.S.: Mutants of yeast with temperature-sensitive isoleucyl-tRNA-synthetases. Proc. nat. Acad. Sci. (Wash.) 59, 422–428 (1968)
Hütter, R., Poralla, K., Zachau, H.G., Zähner, H.: Über die Wirkungsweise von Borrelidin. Biochem. Z. 344, 190–196 (1966)
Magni, G.E.: The origin of spontaneous mutations during meiosis. Proc. nat. Acad. Sci. (Wash.) 50, 975–980 (1963)
McLaughlin, C.S., Hartwell, L.H.: A mutant of yeast with a defective methionyl-tRNA-synthetase. Genetics 61, 557–655 (1969)
Mortimer, R.K., Hawthorne, D.C.: Yeast genetics. In: The yeasts (A.H. Rose and J.S. Harrison, eds.), Vol. 1, pp. 385–460. London and New York: Academic Press 1969
Nass, G.: Regulation of histidine biosynthetic enzymes in a mutant of E. coli with an altered histidyl-tRNA-synthetase. Molec. gen. Genet. 100, 216–224 (1967)
Nass, G., Poralla, K.: Borrelidin resistant mutants of Saccaromyces cerivisiae with an altered threonyl-tRNA-synthetase. Abstract p. 98, 2nd Intern. Symp. “Genetics of Industrial Microorganisms” Sheffield, 1974
Nass, G., Thomale, J.: Alteration of structure or level of threonyl-tRNA-synthetase in borrelidin resistant mutants of E. coli. FEBS Letters 39, 182–186 (1974)
Neidhardt, F.C., Parker, J., McKeever, W.G.: Function and regulation of aminoacyl-tRNA-synthetases in procaryotic and eucaryotic cells. Ann. Rev. Microbiol. 29, 215–250 (1975)
Oxender, D.L.: Amino acid transport in microorganisms. In: Metabolic pathways (L.E. Hokin, ed.), Vol. VI, pp. 133–185. New York: Academic Press 1972
Paetz, W., Nass, G.: Biochemical and immunological characterization of threonyl-tRNA-synthetases of two borrelidin resistant mutants of E. coli K12. Europ. J. Biochem. 35, 331–337 (1973)
Perkins, D.D.: The detection of linkage in tetrad analysis. Genetics 38, 187–197 (1949)
Poralla, K.: Borrelidin. In: Antibiotics (J.W. Corcoran and F.E. Hahn, eds.), Vol. 3, pp. 365–369. Berlin-Heidelberg-New York: Springer 1975
Rasse-Messenguy, F., Fink, G.R.: Feedback resistant mutants of histidine biosynthesis in yeast, p. 85–94, In: Genes, proteins and populations (A.M. Srb, ed.), Vol. 2 of the series: Basic life sciences (A. Hollaender, ed.). New York: Plenum Press 1973
Robichon-Szulmajster, H. de, Surdin, Y., Mortimer, R.K.: Genetic and biochemical studies of genes in Saccharomaces controlling the synthesis of threonine and methionine. Genetics 53, 609–619 (1966)
Sherman, F., Fink, F.R., Lawrence, C.W.: Laboratory manual methods in yeast genetics. Cold Spring Harbor, N.Y., 1971
Surdin-Kerjan, Y., Cherest, H., Robichon-Szulmajster, H. de: Relationship between methionyl-transfer ribonucleic acid cellular content and synthesis of methionine enzymes in Saccharomyces cerivisiae. J. Bact. 113, 1156–1160 (1973)
Author information
Authors and Affiliations
Additional information
Communicated by F. Gros
Rights and permissions
About this article
Cite this article
Nass, G., Poralla, K. Genetics of borrelidin resistant mutants of Saccharomyces cerivisiae and properties of their threonyl-tRNA-synthetase. Molec. gen. Genet. 147, 39–43 (1976). https://doi.org/10.1007/BF00337933
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00337933