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The inhibitory properties of new non-protein amino acids and the screening of effective analogs of L-amino acids for the selection of highly active strain-producers

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Abstract

We studied the antimicrobial properties of 32 new non-protein compounds synthetized in the Scientific and Production Center “Armbiotechnology” by the method of asymmetric synthesis; new analogs of L-histidine and L-isoleucine were found. Via chemical mutagenesis, we obtained new mutants of the wild strain of Brevibacterium flavum 14067 resistant to (S)-β-[4-phenyl-3-(3’-hydroxypropyl)-5-thioxo-1,2,4-thiazole-1-yl]-α-alanine (an analog of histidine) and to (2S,3R)-oxyleucine (an analog of isoleucine). It was found that the synthesis rate of histidine and isoleucine in some mutants is higher than that of most active strain-producers resistant to one of these amino acids analogs described in literature. Our data prove the efficacy of the use of new analogs as selective agents for the screening of highly active strains producing histidine and isoleucine.

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Authors and Affiliations

  1. Scientific and Production Center “Armbiotechnology”, National Academy of Sciences, Yerevan, 0056, Republic of Armenia

    A. Kh. Chakhalyan, S. Gh. Keleshyan, Zh. V. Karapetyan, A. G. Davtyan, G. Y. Avetisova, L. H. Melkonyan, A. S. Dadayan & A. S. Saghyan

Authors
  1. A. Kh. Chakhalyan
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  2. S. Gh. Keleshyan
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  3. Zh. V. Karapetyan
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  4. A. G. Davtyan
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  5. G. Y. Avetisova
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  6. L. H. Melkonyan
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  7. A. S. Dadayan
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  8. A. S. Saghyan
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Corresponding author

Correspondence to A. Kh. Chakhalyan.

Additional information

Original Russian Text © A.Kh. Chakhalyan, S.Gh. Keleshyan, Zh.V. Karapetyan, A.G. Davtyan, G.Y. Avetisova, L.H. Melkonyan, A.S. Dadayan, A.S. Saghyan, 2016, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2016, Vol. 52, No. 1, pp. 62–67.

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Chakhalyan, A.K., Keleshyan, S.G., Karapetyan, Z.V. et al. The inhibitory properties of new non-protein amino acids and the screening of effective analogs of L-amino acids for the selection of highly active strain-producers. Appl Biochem Microbiol 52, 51–55 (2016). https://doi.org/10.1134/S0003683816010038

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  • DOI: https://doi.org/10.1134/S0003683816010038

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