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Yeast nuclear gene CBS2, required for translational activation of cytochrome b, encodes a basic protein of 45 kDa

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Summary

In yeast, synthesis of apocytochrome b from mitochondrial COB mRNA depends on at least three nuclear gene products. The translation stimulatory effect by two of these nuclear genes, CBS1 and CBS2, is mediated by the 5′-untranslated leader of COB mRNA. In this report, we show that CBS2 is located on chromosome IV and provide genetic evidence that the CBS2 gene encodes a polypeptide. Determination of the DNA sequence reveals a contiguous open reading frame of 1167 bp. The deduced polypeptide has a calculated molecular weight of 44.5 kDa and is characterized by a high content of positively charged amino acids. It has no significant homology to any known protein. The CBS2 gene is transcribed into low abundance mRNA species with a major transcription initiation site located 97 bp upstream from the ATG start codon next to a poly(dA-dT) stretch.

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Author notes

  1. Gerhard Rödel

    Present address: Labor für Molekulare Biologie und Allgemeine Pathologie, Institut für Pathologie und Rechtsmedizin der Universität Ulm, Neuherbergstrasse 11, 8000, München 45, Federal Republic of Germany

Authors and Affiliations

  1. Institut für Genetik und Mikrobiologie der Universität München, Maria-Ward-Strasse 1a, D-8000, München 19, Federal Republic of Germany

    Uwe Michaelis, Tobias Schlapp & Gerhard Rödel

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  1. Uwe Michaelis
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  2. Tobias Schlapp
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  3. Gerhard Rödel
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Communicated by R.G. Herrmann

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Michaelis, U., Schlapp, T. & Rödel, G. Yeast nuclear gene CBS2, required for translational activation of cytochrome b, encodes a basic protein of 45 kDa. Mol Gen Genet 214, 263–270 (1988). https://doi.org/10.1007/BF00337720

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