Abstract
Chronic administration of a relatively low concentration of vanadate to rats causes inhibition of water, alpha-aminoisobutyric acid (AIB) and l-alanine absorption. The mechanism responsible for this inhibition was tested by studying the uptake of alanine in isolated rat intestinal cells. The studies suggest that the vanadate inhibition of amino acid transport is primarily caused by a decreased activity of the Na=-K= pump, an action that is similar to what is observed when the rat intestine is acutely exposed to vanadate. Vanadate appeared to have no direct effect on the entry of amino acids into the intestinal cell. This was evident by the fact that amino acid uptake by enterocytes of control rats was not different from the uptake by cells of vanadate-treated animals that have an inwardly directed Na gradient artificially created across them. Furthermore, 86RB influx and efflux into and out of intestinal tissues of the vanadate-treated animals were, respectively, decreased and increased as compared to normal control tissues and they were similar to what is observed when the intestine is acutely exposed to ouabain, a known specific inhibitor of the Na=-K= pump.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Barrabin M, Garrahan PJ, Rega AF (1980) Vanadate inhibition of the Ca2=-ATPase from human red cell membranes. Biochim Biophys Acta 600: 796–804
Beauge LA, Glynn IM (1978) Commercial ATP containing traces of vanadate alters the response of (Na=-K=)ATPase to external potassium. Nature 272: 551–552
Bond GH, Hudgins PM (1980) Inhibition of red cell Ca2=-ATPase by vanadate. Biochim Biophys Acta 600: 781–790
Bowen HJM (1966) Vanadium. In: Bowen HJM (ed) Trace elements in biochemistry. Academic Press, London, UK, pp 207–208
Cantley LC, Josephson L, Warner M, Yanagisawa C, Lechene C, Guidotti G (1977) Vanadate is a potent (NaK)ATPase inhibitor found in ATP derived from muscle. J Biol Chem 252: 7421–7423
Charney AN, Donowitz M (1978) Functional significance of intestinal Na=-K=-ATPase: in vivo ouabain inhibition. Am J Physiol 234: E629-E636
Curran PF, Hajjar JJ, Glynn IM (1970) The sodium-alanine interaction in rabbit ileum: Effect of alanine on sodium fluxes. J Gen Physiol 55: 297–308
Desousa RC, Grosso A (1979) Vanadate blocks cyclic AMP-induced stimulation of sodium and water transport in amphibian epithelia. Nature (London) 279: 803–804
Faller LD, Rabon E, Sachs G (1983) Vanadate binding to the gastric H,K-ATPase and inhibition of the enzyme's catalytic and transport activities. Biochemistry 22: 4676–4685
Fanestil DD (1980) Vanadate: Nonselective inhibition of transepithelial transport of Na=, H= and water. Experientia 36: 1045–1046
Goldberg ED, Griffin JJ, Hodge V, Koide M, Windom H (1979) Pollution history of the Savannah River Estuary. Environ Sci Technol 13: 588–594
Hajjar JJ, Khuri RN, Curran PF (1972) Alanine efflux across the serosal border of turtle intestine. J Gen Physiol 60: 720–734
Hajjar JJ, Khuri RN, Bikhazi AB (1975) Effect of bile salts on amino acid transport by rabbit intestine. Am J Physiol 229: 518–823
Hajjar JJ, Murphy DM, Scheig RL (1979) Mechanism of inhibition of alanine absorption by Na ricinoleate. Am J Physiol 236: E534-E538
Hajjar JJ, Tomicic T, Scheig RL (1981) Effect of chronic ethanol consumption on leucine absorption in the rat small intestine. Digestion 22: 170–176
Hajjar JJ, Zakko S, Tomicic TK (1986) Effects of vanadate on water and electrolyte transport in rat jejunum. Biochim Biophys Acta 863: 325–328
Hajjar JJ, Fucci JC, Rowe WA, Tomicic TK (1987) Effect of vanadate on amino acid transport in rat jejunum. Proc Soc Exp Biol Med 184: 403–409
Jaffe D, Walters JK (1977) Intertidal trace metal concentrations in some sediments from the Humber Estuary. Sci Total Environ 7: 1–15
Kobayashi T, Martensen T, Nath J, Flavin M (1978) Inhibition of dynein ATPase by vanadate, and its possible use as a probe for the role of dynein in cytoplasmic motility. Biochem Biophys Res Commun 81: 1313–1318
Lopez V, Stevens T, Lindquist RN (1976) Vanadium ion inhibition of alkaline phosphatase-catalyzed phosphate ester hydrolysis. Arch Biochem Biophys 175: 31–38
Nechay BR, Saunders JP (1978) Inhibition by vanadium of sodium and potassium dependent adenosine triphosphatase derived from animal and human tissues. J Environ Pathol Toxicol 2: 247–262
Sachs G (1980) Vanadate as a transport probe. J Lab Clin Med 96: 379–381
Schroeder HA, Balassa JJ, Tipton IH (1963) Abnormal trace metals in man-vanadium. J Chronic Dis 16: 1047–1071
Schultz SG, Zalusky R (1964) Ion transport in isolated rabbit ileum II. The interaction between active sodium and active sugar transport. J Gen Physiol 47: 1043–1059
Schultz SG, Curran PF, Chez RA, Fuisz RE (1967) Alanine and sodium fluxes across mucosal border of rabbit ileum. J Gen Physiol 50: 1241–1260
Underwood EJ (1971) Vanadium. In: Underwood EJ (ed) Trace elements in human and animal nutrition. Academic Press, London, UK, pp 416–424
Van Etten RL, Waymack PP, Rehkop DM (1974) Transition metabolism inhibition of enzyme-catalyzed phosphate ester displacement reactions. J Am Chem Soc 96: 6782–6785
Wahawisan R, Richards NW, Gaginella TS, Wallace J (1986) Effects of phenothiazines on phosphate incorporation and chloride uptake in isolated intestinal epithelial cells. Gen Pharmacol 17: 275–280
Weiser MM (1973) Intestinal epithelial cell surface membrane glycoprotein synthesis. I. An indicator of cellular differentiation. J Biol Chem 248: 2536–2541
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hajjar, J.J., Dobish, M.P. & Tomicic, T.K. Effect of chronic vanadate ingestion on amino acid and water absorption in rat intestine. Arch Toxicol 63, 29–33 (1989). https://doi.org/10.1007/BF00334630
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00334630