Summary
Recombinant plasmids having PstI fragments of P22 DNA inserted in the vector pBR322 can be transduced efficiently by Salmonella phage P22, irrespective of the cloned phage sequences. When the rec function of the donor cells and the corresponding recombination system erf of the infecting phage are simultaneously inactivated, only plasmids containing the P22 pac site can be transduced. By this selective, generalized transduction an EcoRV DNA fragment of the P22 related phage L has been identified that carries a base sequence recognized by phage P22 as a packaging signal. Experiments in which only one of the two recombination systems was inactivated, showed that the bacterial rec system obviously promotes cointegrate formation between plasmid and phage DNA much more efficiently than the phage-coded erf system, allowing the specialized plasmid transduction observed by Orbach and Jackson (1982).
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References
Bezdek M, Amati P (1967) Properties of P22 and a related Salmonella typhimurium phage. I. General features and host specificity. Virology 31:272–278
Birnboim HC, Doly J (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucl Acid Res 7:1513–1523
Botstein D, Matz MJ (1970) A recombination function essential to the growth of bacteriophage P22. J Mol Biol 54:417–440
Canosi U, Lüder G, Trautner TA (1982) SPP1-mediated plasmid transduction. J Virology 44:431–436
Casjens S, Huang WM (1982) Initiation of sequential packaging of bacteriophage P22 DNA. J Mol Biol 157:287–298
Chelala CA, Margolin P (1974) Effects of deletions on cotransduction linkage in Salmonella typhimurium: Evidence that bacterial chromosome deletion affect the formation of transducing DNA fragments. Mol Gen Genet 131:97–112
Chisholm RL, Deans RJ, Jackson EN, Jackson DA, Rutila JE (1980) A physical gene map of the bacteriophage P22 late region: Genetic analysis of cloned fragments of P22 DNA. Virology 102:172–189
Cohen SN, Chang AC, Hsu L (1972) Nonchromosomal antibiotic resistance in bacteria: Genetic transformation of Escherichia coli by R-factor DNA. Proc Natl Acad Sci USA 69:2110–2114
Ebel-Tsipis J, Botstein D, Fox MS (1972) Generalized transduction by phage P22 in Salmonella typhimurium. I. Molecular origin of transducing DNA. J Mol Biol 71:433–448
Fuke M, Inselburg J (1972) Electron microscope studies of replicating and catenated colicin factor E1 DNA isolated from mini-cells. Proc Natl Acad Sci USA 69:89–92
Ish-Horowicz D, Burke JF (1981) Rapid and efficient cosmid cloning. Nucl Acid Res 9:2989–2998
Jackson EN, Jackson DA, Deans RJ (1978) EcoRI analysis of bacteriophage P22 DNA packaging. J Mol Biol 118:365–388
Kufer B, Backhaus H, Schmieger H (1982) The packaging initiation site of phage P22. — Analysis of packaging events by transduction. Mol Gen Genet 187:510–515
Orbach MJ, Jackson EN (1982) Transfer of chimeric plasmids among Salmonella typhimurium strains by P22 transduction. J Bacteriol 149:985–994
Ozeki H (1959) Chromosome fragments participating in transduction in Salmonella typhimurium. J Bacteriol 117:457–470
Raj AS, Raj AY, Schmieger H (1974) Phage genes involved in the formation of generalized transducing particles in Salmonella-phage P22. Mol Gen Genet 135:175–184
Rutila JE, Jackson EN (1981) Physical map of the bacteriophage P22 genome. Virology 113:769–775
Schmieger H (1970) The molecular structure of the transducing particles of Salmonella-phage P22. II. Density gradient analysis of DNA. Mol Gen Genet 109:323–337
Schmieger H (1982) Packaging signals for phage P22 on the chromosome of Salmonella typhimurium. Mol Gen Genet 187:516–518
Schmieger H, Backhaus H (1976) Altered contransduction frequencies exhibited by HT-mutants of Salmonella-phage P22. Mol Gen Genet 143:307–309
Stahl FW (1979) Special sites in generalized recombination. Annu Rev Genet 13:7–24
Takahashi H, Saito H (1982) Mechanism of pBR322 transduction mediated by cytosine-substituting T4 bacteriophage. Mol Gen Genet 186:497–500
Weaver S, Levine M (1977) Recombinational circularization of Salmonella phage P22 DNA. Virology 76:29–38
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Communicated by H. Saedler
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Schmidt, C., Schmieger, H. Selective transduction of recombinant plasmids with cloned pac sites by Salmonella phage P22. Mol Gen Genet 196, 123–128 (1984). https://doi.org/10.1007/BF00334103
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DOI: https://doi.org/10.1007/BF00334103