Abstract
Adult female Wistar rats were fed with a diet containing 0.05% hexachlorobenzene. On the 60th day of this treatment the specific activities of NADPH: Δ 4-3-oxosteroid-5α-reductase and the 3-hydroxysteroid dehydrogenases in rat liver microsomes were diminished compared to control rats. The cytoplasmatic 5β-reduction was higher in HCB treated rats than in control rats. These alterations of the steroid metabolism lead to increased formation of 5β-H-steroids which are known to be inducers of the porphyrin biosynthesis.
Zusammenfassung
Erwachsene weibliche Wistar-Ratten wurden mit 0.05% Hexachlorbenzol enthaltendem Futter ernährt. Am 60. Tag dieser Behandlung waren die spezifischen Aktivitäten der NADPH: Δ 4-3-oxosteroid-5α-Reduktase und der 3-Hydroxysteroid-Dehydrogenasen in Lebermikrosomen, verglichen mit Kontroll-Ratten, vermindert. Die cytoplasmatische 5β-Reduktion war bei HCB-behandelten Ratten höher als bei Kontroll-Ratten. Diese Veränderungen des Steroidmetabolismus führen zu vermehrter Bildung von 5β-H-Steroiden, von denen bekannt ist, daß sie Induktoren der Porphyrin-Biosynthese sind.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- HCB:
-
Hexachlorobenzene
- 5α-DHT:
-
5α-Dihydrotestosterone (17β-hydroxy-5α-androstan-3-one)
- 5β-DHT:
-
5β-dihydrotestosterone (17β-hydroxy-5β-androstan-3-one)
References
Doss, M., Schmid, A.: Rapid determination of urinary total porphyrin by ion exchange chromatography. Z. Klin. Chem. Klin. Biochem. 9, 415–420 (1971)
Doss, M., Schmid, A.: Quantitative Bestimmung von δ-Aminolaevulinsäure und Porphobilinogen im Urin mit Ionenaustauscherchromatographie-Fertigsäulen. Z. Klin. Chem. Klin. Biochem. 9, 99–102 (1971)
Ghraf, R., Hoff, H. H., Lax, E. R., Schriefers, H.: The 5β-metabolites of testosterone: mode and sex-specificity of their formation in the rat liver. Acta Endocrinol. (Kbh.) 73, 577–584 (1973)
Goerz, G., Krieg, Th., Bolsen, K., Lissner, R.: Langzeit-Exposition von Ratten mit Hexachlorbenzol (HCB): Einfluß auf die Porphyrin-Ausscheidung im Urin und auf das Cytochrom P-450 in der Leber. Arch. Dermatol. Res. 259, 199–206 (1977)
Goldberg, A., Moore, M. R., Beattie, A. D., Hall, P. E., McCallum, J., Grant, J. K.: Excessive urinary excretion of certain porphyrinogenic steroids in human acute intermittent porphyria. Lancet 1969 I, 115–118
Golf, S. W., Graef, V., Staudinger, Hj.: Untersuchungen zum Mechanismus der Δ 4-3-Oxosteroid-5α-Reduktion in Rattenleber-Mikrosomen. Hoppe-Seyler's Z. Physiol. Chem. 355, 1499–1507 (1974)
Golf, S. W., Graef, V., Nowotny, E.: Solubilisierung und Anreicherung einer 3α-Hydroxysteroid-Dehydrogenase aus Rattenleber-Mikrosomen. Hoppe-Seyler's Z. Physiol. Chem. 357, 35–40 (1976)
Golf, S. W., Graef, V.: Isolation of a 3-equatorial-hydroxysteroid dehydrogenase from rat liver microsomes. FEBS-Lett. 64, 315–318 (1976)
Golf, S. W., Graef, V.: Isolation of a NAD: 3β(α)-hydroxy-5β-androstane dehydrogenase from rat liver microsomes. J. Steroid Biochem. 10, 201–205 (1979)
Graef, V., Golf, S. W.: Wirkung von 6β-Methoxy-10α-pregna-4,6-dien-3,20-dion (Ro 6-1963) auf die Steroidreduktasen in der Rattenleber. Experientia 32, 1250–1251 (1976)
Graef, V., Golf, S. W., Rempeters, G.: Solubilization of NADH: 4-ene-3-oxosteroid-5α-reductase from rat-liver microsomes. J. Steroid Biochem. 9, 369–371 (1978)
Granick, S., Kappas, A.: Steroid induction of porphyrin synthesis in liver cell culture. I. Structural basis and possible physiological role in the control of heme formation. J. Biol. Chem. 242, 4587–4593 (1967)
Gustafsson, J.-Å., Ingelman-Sundberg, M.: Changes in steroid hormone metabolism in rat liver microsomes following administration of 2,3,7,8-tetrachlorodibenzo-p-dioxine (TCDD) Biochem. Pharmacol. 28, 497–499 (1979)
Kappas, A., Bradlow, H. L., Bickers, D. R., Alvares, A, P.: Induction of a deficiency of steroid Δ 4-5α-reductase activity in liver by a porphyrinogenic drug. J. Clin. Invest. 59, 159–164 (1977)
Leybold, K., Staudinger, Hj.: Phosphat und Arsenat als Co-Faktoren der DPNH-abhängigen mikrosomalen Hydrierung von Δ 4-3-Ketosteroiden in vitro. Biochem. Z. 337, 320–327 (1963)
Ockner, R. K., Schmid, R.: Acquired porphyria in man and rat due to hexachlorobenzene intoxication. Nature 189, 499 (1961)
Robel, P., Emiliozzi, R., Baulieu, E. E.: Studies on testosterone metabolism. III. The selective “5β-metabolism” of testosterone glucuronide. J. Biol. Chem. 241, 20–29 (1966)
Author information
Authors and Affiliations
Additional information
Dedicated to Prof. Dr. Hj. Staudinger on occasion of his 65th birthday
Rights and permissions
About this article
Cite this article
Graef, V., Golf, S.W. & Goerz, G. Effect of hexachlorobenzene on enzymes of the steroid metabolism in rat liver. Arch Toxicol 43, 115–120 (1979). https://doi.org/10.1007/BF00333618
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00333618