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Retinol (vitamin A) as a modifier of 2-aminofluorene and 2-acetyl-aminofluorene mutagenesis in the Salmonella/microsome assay

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Abstract

Vitamin A (retinol) has been demonstrated to modify the mutagenic activity of the aromatic amines, 2-aminofluorene (2AF) and 2-acetylaminofluorene (2AAF) when added to the Ames Salmonella/mammalian microsome assay. Low amounts of retinol (2–20 μg/plate) increased the mutagenicity of both 2AF and 2AAF. At higher doses (50–150 μg/plates) the mutagenicity of 2AAF remained unchanged while the mutagenicity of 2AF gradually decreased.

The present data do not support the hypothesis that retinol generally acts as an inhibitor of in vitro metabolic activation of procarcinogens.

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Abbreviations

2AF:

2-aminofluorene

2AAF:

2-acetylaminofluorene

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Authors and Affiliations

  1. Toxicology Laboratory, National Food Administration, Box 622, S-751 26, Uppsala

    Leif Busk

  2. Department of General Genetics, University of Uppsala, S-750 07, Uppsala

    Leif Busk

  3. Unit of Toxicology, National Institute of Environmental Medicine, Karolinska Institute, S-10401, Stockholm, Sweden

    Ulf G. Ahlborg

Authors
  1. Leif Busk
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  2. Ulf G. Ahlborg
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Busk, L., Ahlborg, U.G. Retinol (vitamin A) as a modifier of 2-aminofluorene and 2-acetyl-aminofluorene mutagenesis in the Salmonella/microsome assay. Arch Toxicol 49, 169–174 (1982). https://doi.org/10.1007/BF00332364

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  • DOI: https://doi.org/10.1007/BF00332364

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