Abstract
We sought for cloned sequences of middle repetitive (MR) complexity that mark obligatory heterochromatic regions. Total genome probes were employed in a differential screening procedure to recover X-specific, Y-specific and autosomal specific heterochromatic sequences. X- and Y-linked sequences were recovered in the same experiment. (Y-linked clones will be described elsewhere). All nine independent, non-identical X-specific clones were found to be partially homologous to one another and to type I rDNA insertion. No other X-specific Bam HI or HindIII clones were found. In situ hybridization to normal and inverted chromosomes revealed extensive homology in the heterochromatin spanning the nucleolus organizer (NOR) and the eu-heterochromatin junction. Eleven clones which are underrepresented in polytene chromosomes were selected in another differential screening. None was autosome-specific. Five were of nucleolar origin. Among them a presumptive type II 28SrDNA insertion sequence was clearly localized within the X-chromosome proximal heterochromatin in addition to the known localization of the X and Y nucleolar organizers. We mapped three clones to major sites on the Y chromosome and to secondary autosomal sites. The results are discussed with regard to the complexity of heterochromatin organization.
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References
Atherton, D., Gall, J.G.: Salivary gland squashes for in situ nucleic acid hybridization studies. Dros. Inf. Serv. 149, 131–133 (1972)
Becker, H.J.: The influence of heterochromatin inversion heterozygosity and somatic pairing on X-ray induced mitotic recombination in Drosophila melanogaster. Molec. Gen. Genet. 105, 203–218 (1969)
Bolivar, F., Rodriguez, R.L., Green, P., Betach, M.C., Boger, H.W., Crosa, J., Falkav, S.: Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene 2, 95–113 (1977)
Bonner, J.J., Pardue, M.L.: Ecdjson-stimulated RNA synthesis in imaginal discs of Drosphila melanogaster: Assay by in situ hybridization. Chromosoma (Berl.) 58, 87–99 (1976)
Brosseau, G.E., Nicoletti, B., Grell, E.H., Lindsley, D.L.: Production of altered Y chromosomes bearing specific sections of the X-chromosome in Drosophila. Genetics 46, 339–346 (1961)
Brutlag, D.: Molecular arrangement and evolution of heterochromatic DNA. Ann. Rev. Genet. 14, 121–144 (1980)
Carlson, M., Brutlag, D.: A gene adjacent to satellite DNA in Drosophila melanogaster. Proc. Natl. Acad. Sci. (Wash.) 75, 5898–5902 (1978)
Cooper, K.W.: Cytogenetic analysis of major heterochromatic elements (especially Xh and Y) in Drosophila melanogaster and the theory of “heterochromatin”. Chromosoma (Berl.) 10, 535–588 (1959)
Davis, R.W., Simon, M., Davidson, N.: Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids. In: Methods in enzymology (L. Grossman, K. Moldave, eds.) vol. 21D, pp. 413–428, New York: Academic Press 1971
Dawid, I.B., Botchan, P.: Sequences homologous to ribosomal insertions occur in the Drosophila genome outside the nucleolus organizer. Proc. Natl. Acad. Sci. (Wash.) 74, 4233–4237 (1972)
Dawid, I.B., Lauth, M., Wellauer, P.K.: Repetitive DNA elements related to the ribosomal insertion of Drosophila melanogaster. In: Mobilization and reassembly of genetic information (W.B. Scott, R. Werner, D.R. Joseph, eds.) Miami Winter Symp. 17, 217–234 (1980)
Dawid, I.B., Long, E.O., Di Novera, P.P., Pardue, M.L.: Ribosomal insertion like elements in Drosophila melanogaster are interspersed with mobile sequences. Cell 25, 399–408 (1981)
Gall, J.G., Cohen, E.H., Polan, M.L.: Repetitive DNA sequences in Drosophila. Chromosoma (Berl.) 33, 319–344 (1971)
Grunstein, M., Hogness, D.S.: Colony hybridization: A method for the isolation of cloned DNAs that contain a specific gene. Proc. Natl. Acad. Sci. (Wash.) 72, 3961–3965 (1975)
Hanna, A.: Localization and function of heterochromatin in Drosophila melanogaster. Advanc. Genet. 4, 87–125 (1951)
Hennig, W., Meer, B.: Reduced polyteny of ribosomal RNA cistrons in giant chromosomes of Drosophila hydei. Nature (Lond.) New Biology 233, 70–72 (1971)
Holmquist, G.: Hoechst 33258 fluorescent staining of Drosophila chromosomes. Chromosoma (Berl.) 49, 333–356 (1975)
Kidd, S., Glover, D.M.: A DNA segment from Drosophila melanogaster which contains five tandemly repeating units homologous to the major rDNA insertion. Cell 19, 103–119 (1980)
Lifschytz, E.: A procedure for the cloning and identification of Y specific middle repetitive sequences in Drosophila hydei. J. Mol. Biol. 133, 267–277 (1979)
Lindsley, D.L., Grell, E.H.: Genetic variation of Drosophila melanogaster Carnegie Inst. Wash. Publ. 627, p. 471 (1968)
Maniatis, T., Jefrey, A., Kleid, D.: Nucleotide sequence of the rightward operator of phage λ. Proc. Natl. Acad. Sci. (Wash.) 72, 1184–1188 (1975)
Pardue, M.L., Gall, J.G.: Nucleic acid hybridization to the DNA of cytological preparations. In: Methods in cell biology (D. Prescott, ed) vol. 10, pp. 1–16. New York: Academic Press, 1975
Peacock, W.J., Lake, A.R., Gerlach, W.L., Dunsmuir, P., Dennis, E.S., Appels, R.: Fine structure and evolution of DNA in heterochromatin. Cold Spring Harb. Symp. Quant. Biol. 42, 1121–1135 (1978)
Peacock, W.J., Appels, R., Endow, S., Glover, D.M.: Chromosomal distribution of the major inserts in D. melanogaster 28S ribosomal RNA genes. Genet. Res. 37, 209–214 (1981)
Roiha, H.J., Glover, D.M.: Characterization of complete type II insertions in cloned segments of ribosomal DNA from Drosophila melanogaster. J. Mol. Biol. 140, 351–355 (1980)
Roiha, H.J., Miller, R.J., Woods, L.C., Glover, D.: Arrangements and rearrangements of the sequences flanking the two types of rDNA insertions in D. melanogaster. Nature (Lond.) 290, 749–753 (1981)
Rudkin, G.T.: The structure and function of heterochromatin. In: Genetics Today (S.J. Geerts, ed.), vol. 2, pp. 359–375 New York: Pergamon Press 1964
Southern, E.M.: Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98, 503–517 (1975)
Spear, B., Gall, J.G.: Independent control of ribosomal gene replication in polytene chromosomes of Drosophila melanogaster. Proc. Natl. Acad. Sci. (Wash.) 70, 1359–1363 (1973)
Tartof, K.D., Dawid, I.B.: Similarities and differences in the structure of X and Y chromosome rRNA genes in Drosophila. Nature (Lond.) 263, 27–30 (1976)
Ullrich, A., Shine, J. Chirgwin, J., Pictet, R., Tischer, E., Rutter, W.J., Goodman, H.M.: Rat insulin genes: Construction of plasmids containing the coding sequence. Science 196, 1313–1319 (1977)
Young, M.W.: Middle repetitive DNA: A fluid component of the Drosophila genome. Proc. Natl. Acad. Sci. (Wash.) 76, 6274–6278 (1979)
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Lifschytz, E., Hareven, D. Heterochromatin markers: A search for heterochromatin specific middle repetitive sequences in Drosophila . Chromosoma 86, 429–442 (1982). https://doi.org/10.1007/BF00330119
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DOI: https://doi.org/10.1007/BF00330119